摘要
目的:探讨人端粒酶逆转录酶(hTERT)启动子调控Bax基因在端粒酶阳性的腺样囊性癌细胞-SACC-83中表达的特异性,以及Bax对SACC-83细胞增殖的影响。方法:脂质体介导pACTERT-Bax质粒载体转染SACC-83和端粒酶阴性的人胚肺成纤维细胞(HEL),通过RT-PCR检测Bax表达,MTT法检测Bax基因表达对细胞增殖的影响,流式细胞仪检测细胞凋亡率。结果:hTERT启动子调控Bax基因在SACC-83细胞中明显表达,对该肿瘤细胞的增殖有明显的抑制作用(相对活性58.90%),且诱导细胞凋亡比例增加(11.62%);而在HEL细胞中hTERT启动子未能诱导Bax基因表达,对该细胞的增殖和凋亡无明显影响。结论:hTERT启动子可以诱导Bax基因在SACC-83中特异性表达及诱导细胞凋亡,同时还能消除Bax基因对正常细胞的潜在毒性。
OBJECTIVE： To investigate the expression specificity of gene Bax driven by human telomerase reverse transcriptase （hTERT） promoter in adenvid cystic carcinoma cells （SACC-83） with telomerase activity and the growth inhibitory capability of Bax to SACC-83. METHODS： After transfecting pACTERT-Bax plas- mid into SACC-83 cells and HEL cells through liposome, its effect on these ceils was investigated in vitro by using RT-PCR technique, MTT method and flow cytometry. RESLUTS： The expression of extrinsic gene Bax driven by hTERT promoter was detected in SACC- 83 cells with telomerase activity, and not detected in HEL cells without telomerase activity. After transfection of pACTERT-Bax, the proliferation of SACC-83 cells showed a significant inhibitory effect （the relative cell viability was 58.90 % ）, and its apoptotic rate was promoted （11.62%）, whereas no inhibitory effect was observed in HEL cells and its apoptotic rate showed little change. CONCLUSION： hTERT promoter can be used to induce tumor-specific expression of Bax and apoptosis in SACC-S3 cells, and avoid potential toxicity of Bax to normal somatic cells.
出处
《中华肿瘤防治杂志》
CAS
2009年第12期902-905,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
浙江省湖州市2007年自然科学基金(2007YZ04)
关键词
癌
腺样囊性
基因表达
细胞凋亡
细胞系
肿瘤
carcinoma, adenoid cystic
gene expression
apoptosis
cell line, tumor
