摘要
以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方:⑴芽诱导培养基:MS+BA 0.6 mg/L+NAA 0.2 mg/L+琼脂7.3 g/L+蔗糖30 g/L,pH 5.8;⑵增殖培养基:MS+BA 0.8 mg/L+NAA 0.05 mg/L+琼脂7.3 g/L+蔗糖30 g/L,pH 5.8;⑶生根培养基:MS+BA 0.05 mg/L+NAA 0.8 mg/L+琼脂7.3 g/L+蔗糖30 g/L,pH 5.8.观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15 d,第3种培养基与前人的相当,比其快2 d.
The optimal culture mediums were obtained by tissue culture method from Induction of bud, culture of multiplication and culture of root of taking Lanzhou Lily as a exophyte. They were： （1） the culture medium of bud induction： MS＋ BA 0.6 mg/L＋NAA 0.2 mg/L＋agar 7. 3 g/L＋ sucrose 30 g/L, pH 5. 8. （2） the culture medium of multiplication： MS＋BA 0.8 mg/L＋NAA 0.05mg/L＋agar 7.3 g/ L＋sucrose 30 g/L,pH 5.8. （3） the culture medium of rooting culture ： MS＋ BA 0.05 mg/IL＋NAA 0.8 mg/L＋agar 7.3 g/L＋sucrose 30 g/L, pH 5.8. Compared with existing ones, the multiplication rate of the former two ones were faster 15 days, the third culture medium was faster 2 days.
出处
《天津师范大学学报（自然科学版）》
CAS
北大核心
2009年第3期62-65,共4页
Journal of Tianjin Normal University：Natural Science Edition
关键词
兰州百合
鳞片
组织培养
Lanzhou Lily
scales
tissue culture
