摘要
目的建立基于常染色体STR基因座共有等位基因数的全同胞关系判别标准。方法根据280对全同胞及2003对无关个体Identifiler系统15个STR基因座的分型结果,对15个STR基因座的共有等位基因数(S15)和全同胞指数(FSI)进行统计,应用SAS8.2软件包得出Fisher判别函数并与ITO法结果进行比较。结果全同胞对及无关个体对中共有等位基因数目均符合正态分布。采用Identifiler系统15个STR基因座共有等位基因数进行全同胞关系判别时,判别函数分别为:ZFS=3.26970S15-31.51174和ZUI=1.70058S15-8.52411。用上述判别函数进行全同胞/无关个体关系判别时的平均错判率为0.0298。15个STR基因座共有等位基因数法、CODIS13个STR基因座共有等位基因数法与ITO法判别结果差异无统计学意义。结论应用常染色体STR基因座的共有等位基因数判别全同胞关系简便、可信,易于掌握且不受STR基因座等位基因频率的影响。
Objective To establish and evaluate the method of full sibling identification based on the number of allele shared among autosomal STR Loci. Methods Two hundred and eighty full sibling pairs and 2 003 unrelated individual pairs were genotyped in 15 STR loci with Identifiler Kit, and the number of allele shared among the 15 STR loei（S15） and full sibling index（FSI） were calculated. Fisher discriminant functions were established with SAS 8.2 software based on Sis, the power of which were compared with ITO method. Results The distribution of S15 in full sibling pair group and unrelated individual pair group were in accord with normal distribution. The established Fisher discriminant functions for each group were ZFS= 3.26970S15-31.511 74 and ZUI=1.70058 S15-8.524 11, respectively. The average error of probability in sibling and unrelated pair group was 0.029 8. There was no statistically significant difference on the power of full sibling discriminant between the method based on the number of allele shared among the 15 STR loci or the CODIS 13 STR loci and the ITO method. Conclusion The method based on the number of allele shared among the 15 STR loci in full sibling identification is convenient, credible, easy to handling and unaffected by the allele frequency of STR loci.
出处
《法医学杂志》
CAS
CSCD
2009年第4期267-270,共4页
Journal of Forensic Medicine
基金
中央级科研院所社会公益研究资助项目(GY0604)
