摘要
目的:建立测定湿热痹片中盐酸小檗碱含量的HPLC法。方法:采用高效液相色谱法对湿热痹片中的盐酸小檗碱进行含量测定,色谱柱为迪马Diamonsil(TM)钻石C18柱(4.6mm×250mm,5μm),流动相为乙腈-1%磷酸溶液(48∶52,每100毫升中加入十二烷基磺酸钠0.1g),流速为1.0ml/min,检测波长为265nm,柱温为25℃,进样量为10μl。结果:盐酸小檗碱在0.0186～0.3720μg范围内线性关系良好,r=0.9999;平均回收率为100.14%,RSD为1.35%。结论:本法操作简便、准确、重现性好,可用于湿热痹片的质量控制。
Objective： To establish a HPLC method to determine berbefine hydrochlorde in Shirebi Tablets. Methods： The contents of berberine hydrochlorde were determined in Shirebi Tablets by HPLC. A Diamonsil （TM） Cls chromatographic column（4.6 mm×250 mm, 5μm） was used, the mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution（g8：52） at the flow rate of 1.0 ml/min, the detection wavelength was 265 nm, the column temperature was 25℃ and the sample volume was 10 μl. Results： The linear range of berberine hydrochlorde was 0.018 6-0.372 0 μg （r=0.999 9）, the average recovery was 100.14% with a RSD of 1.35%. Conclusion： The method is easy to operate, and can provide accurate and reproducible results, thus is suitable for the quality control of Shirebi Tablets.
出处
《中国医药导报》
CAS
2009年第23期52-54,共3页
China Medical Herald
