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RP-HPLC同时检测发酵液中7-脱氢胆固醇与胆固醇的研究 被引量:1

Simultaneous determination of 7-dehydrocholesterol and cholesterol in fermented liquid by RP-HPLC
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摘要 7-脱氢胆固醇是维生素D3的直接前体,试验研究了RP-HPLC同时检测发酵液中7-脱氢胆固醇及其前体胆固醇的方法和条件。应用Waters sunfire C18(Φ416×150 mm,5μm)色谱柱,乙腈∶异丙醇=80∶20(v∶v)为流动相,1.0mL/min流速,38℃柱温,于波长210 nm紫外检测器检测。在此条件下,7-脱氢胆固醇和胆固醇呈现较好的分离和重现性,其保留时间分别为8.248min和9.598min左右,最低检测浓度分别为8mg/L和10 mg/L,平均回收率分别为99.53%和97.67%。 7 - dehydrocholesterol (7 - DHC ) is a direct precursor of vitamin D3. In this paper, the method and the conditions of simultaneous determination of 7 - dehydrocholesterol and its precursor cholesterol by reversed phase - high performance liquid chromatography ( RP - HPLC ) were studied. The samples were analyzed on a C18 column ( Waters sunfire C18 , Ф 416 × 150 mm,5 μm) using a mixture of methyl cyanide : isopropanol (80:20, v/v) as the mobile phase, a flow rate of 1.0mlfmin, and the wavelength of 210 nm. Within 0 mg/ml - 0. 1 mg/ml of 7 -DHC and cholesterol, the method was accurate with the average recoveries rate of 99.53% and 97.67%, and the minimum determination limit of 8 mg/L and 10 mg/L, respectively.
出处 《中国酿造》 CAS 北大核心 2009年第8期155-157,共3页 China Brewing
基金 国家重点基础研究发展计划"973计划"(2007CB707802)
关键词 胆固醇 7-脱氢胆固醇 RP—HPLC检测 维生素D前体 cholesterol 7 - dehydrocholesterol RP - HPLC vitamin D3
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参考文献9

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共引文献20

同被引文献18

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