摘要
【目的】了解糖基水解酶62家族细胞壁降解酶系在稻瘟病菌致病中的作用。【方法】通过生物信息学方法对稻瘟病菌基因组中假定的糖基水解酶62家族成员进行基因结构、蛋白分泌特性及系统发育分析,并对其中一个成员MGG_01403.6进行过量表达、基因敲除分析。【结果】该家族共有8个成员,均具有细胞壁降解酶(糖基水解酶62家族)保守结构域,且均为胞外分泌蛋白;系统发育分析可以将这些成员分别聚类在两个进化分支中;成员间在不同侵染阶段表达模式有差异;MGG_01403.6过量表达和基因敲除均不影响稻瘟病菌的致病性。【结论】糖基水解酶62家族可能存在功能冗余作用,进一步的双突变或多突变可能是明确这类多基因家族基因功能的重要方法。
[Objective] Glycosyl hydrolase family 62 encode putative alpha-L-arabinofuranosidases, a group of cell-wall degrading enzymes which may involve in the fungal pathogenesis. To better understand the role of cell-wall degrading enzymes in pathogenesis, the putative glycosyl hydrolase family 62 in Magnaporthe grisea we characterized in this study. [Method] The bioinformaties tools were firstly used to analyze putative members in the annotated glycosyl hydrolase family 62, including their gene structure, protein secretion characters, and conduct a phylogenetic analysis. Then overexpression and knockout techniques were applied to further study the function of MGG01403.6, one of the 8 members in the family. [ Result ] The result showed that all the members are secreted proteins with cell-wall degrading enzyme （Glycosyl hydrolase family 62） domain. Phylogenetic analysis showed that 8 members of glycosyl hydrolase family 62 of the fungus could be classified into 2 clusters. Gene expression showed difference among family members after fungal infection. But gene knockout and overexpression of MGG_01403.6 had no effect on the fungal pathogenicity. [Conclusion] Functional redundancy may exist in glycosyl hydrolase family 62 members from Magnaporthe grisea. The double or multiple deletions are necessary for further characterization of the function of this multi-gene family.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第8期2754-2762,共9页
Scientia Agricultura Sinica
基金
国家自然科学基金(30471132
30671348)
关键词
稻瘟病菌
细胞壁降解酶
糖基水解酶62家族
Magnaporthe grisea
cell-wall degrading enzymes
glycosyl hydrolase family 62
