摘要
【目的】对小麦白粉菌侵染后的叶片进行差异蛋白质组学研究,以期发现抗白粉病基因Pm21转入后,对抗病机制起重要作用的蛋白。【方法】以对小麦白粉病敏感(百农3217)和对小麦白粉病免疫(W2132-6,携带抗病基因Pm21)的2个小麦近等基因系为材料,分别提取2个材料拔节期接种48h后的叶片蛋白,应用双向电泳联合质谱(MALDI-TOF-MS)技术分析Pm21基因转入后差异蛋白表达。【结果】双向电泳后,CBB染色,用ImageMasterTM 2D Platinum软件分析检测出12个差异蛋白点,经过质谱(MALDI-TOF-MS)分析和数据库检索,鉴定出9个蛋白质,其中7个分别与能量代谢、基因调控、防卫和稳定蛋白质相关,参与了增强能量代谢、基因调控、抗氧化、细胞壁加厚和木质化等抗病生理反应。【结论】抗白粉病基因Pm21转入后,材料对白粉菌侵染响应发生变化,脯氨酸富集蛋白等胁迫反应蛋白得到增量表达,这些蛋白可能与小麦抗白粉病有一定相关性。
[Objective] In order to understand differential expression proteins in the response to wheat powder mildew between two near-isogenic wheat lines, BAINONG 3217, which is susceptive to wheat powder mildew, and W2132-6, which carrys resistance gene Pm21 and is resistant to wheat powder mildew. [Method] Differential proteomics between the two lines were studied after they were infected by Blumeria graminis. Protein samples were respectively extracted from the leaves after 48 h inoculation, separated by two-dimensional electrophoresis, dyed by CBB, and were identified by MALDI-TOF-MS. [Result] It was found that there were twelve protein spots expressed differentially, and nine of them were identified by MALDI-TOF-MS and database searching. The predicted function of seven proteins were related to energy metabolism, signal control, defensive responses and proteins stabilization, which were involved in many resistant physiological reactions, including enhancing energy metabolism, signal control, antioxidant reaction, cell-wall reinforcement and lignification. [ Conclusion ] The response to powder mildew was changed by Pm21. Stress response proteins （e.g. proline-rich family protein） should be related with resistance to wheat power mildew.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第8期2778-2783,共6页
Scientia Agricultura Sinica
基金
国家"863"项目(2006AA1021E9)
国家科学基础研究"973"项目(2009CB118301)
