摘要
选用胡萝卜栽培品种“开拓者”和“长城红明”无菌苗的子叶和下胚轴作外植体，对可能影响胡萝卜体细胞胚分化率的基本培养基、激素配比进行了研究。结果表明：开拓者对B5培养基表现适应，长城红明对B5和MS培养基均表现适应。下胚轴外植体诱导体细胞胚的分化能力极显著地高于子叶。低浓度的2，4-D（0．1～0．7mg／L）对体细胞胚的分化有促进作用，6-BA对体细胞胚分化有抑制作用。诱导开拓者下胚轴分化体细胞胚的优化培养基为B5＋2，4-D0．1mg／L，分化率达85％。诱导长城红玥下胚轴分化体细胞胚优化培养基为MS＋2，4-D0．7mg／L，分化率达73．3％。2个品种的下胚轴在各自的优化培养基上分化体细胞胚的能力差异不显著。
This study optimized factors which probablely affected somatic embryogenesis frequency such as basic media, hormone composition in medium by using hypocotyls and cotyledons of Daucus carota L. var. Kaituozhe and Changchenghongyue as explants. The results showed as follow： the suitable basic medium for Kaituozhe was 135,and for Changchenghongyue was B5 as well as MS; the embryoid differentiation frequency of hypocotyls were very significant higher than that of cotyledons;Low density of 2,4-D（0.1-0.7 mg/L） had a promotable effect on somatic embryogenesis, whereas 6-BA had a inhibitory effect on somatic embryogenesis; the optimum combination for inducing Kaituozhe＇s hypocotyls somatic embryogenesis was B5 agar medium with 0.1 mg/L 2,4-D, which differentiation frequency was up to 85 %,and the optimum combination for inducing Changchenghongyue＇s hypocotyls somatic embryogenesis was MS agar medium with 0. 7 mg/L 2,4-D, which differentiation frequency was up to 73. 3%; the embryoid differentiation frequency of both hypocotyls on their optimum medium was no difference.
出处
《北方园艺》
CAS
北大核心
2009年第8期73-77,共5页
Northern Horticulture
基金
黑龙江省自然科学基金资助项目(C0014)
哈尔滨市学科后备带头人基金资助项目(0071007002)
黑龙江省普通高等学校骨干教师创新能力资助项目
关键词
胡萝卜
体细胞胚
组织培养
Carrot
Somatic embryos
Tissue culture
