摘要
以墨兰中的金凤锦为试验材料进行组织培养,研究了不同因素对墨兰根状茎增殖的影响。结果表明:最适于墨兰根状茎增殖的基本培养基为1/2MS;添加2 mg/L BA、0.1 mg/LNAA有利于墨兰根状茎的增殖;添加香蕉汁对墨兰的增殖效果不明显。认为墨兰根状茎增殖的最佳培养基配方为:1/2 MS+BA 2 mg/L+NAA 0.1 mg/L+0.8 g/L活性炭。
Cymboidzium sinensis JinFengJin was used as material to carried out tissue culture. The effects of different factors on multiplication of rhizome of Cymbidium sinensis were analyzed. The results showed that the optimal basic medium for multiplication of rhizome of Cymbidium sinensis was 1/2MS, and 1/2 MS supplemented with 2. 0 mg/L BA＋0.1 mg/L NAA was beneficial for multiplication of rhizome, and 1/2 MS＋2. 0 mg/L BA＋0. 1 mg/L NAA supplemented with banana juice had no obvious proliferative effect. So the optimal medium for multiplication of rhizome of Cymbidium sinensis was 1/2 MS＋BA 2 mg/L＋NAA 0. 1 mg/L＋0.08% activated carbon.
出处
《北方园艺》
CAS
北大核心
2009年第8期208-209,共2页
Northern Horticulture
关键词
墨兰
增殖
培养基
Cymbidium Sinensis
Multiplication
Medium
