摘要
为建立猪链球菌快速诊断方法,本研究以纯化的原核表达的猪链球菌谷氨酸脱氢酶蛋白(GDH)为抗原,建立了检测猪链球菌抗体的间接ELISA诊断方法。实验确定了最佳抗原包被浓度为6μg/mL,待检血清最佳稀释倍数为1∶100,其作用时间为60min,兔抗猪酶标抗体的最佳稀释倍数为1∶2000,其作用时间为60min;判定标准为S/P值≥0.210时判为阳性,S/P值≤0.166时判为阴性,介于两者之间的判为可疑。实验结果表明该方法特异性、敏感性和重复性均较好,适用于猪链球菌所有亚型的抗体检测,为猪链球菌的流行病学调查提供了一种简便快速的血清学检测方法。
An indirect-ELISA for detection of antibody against Streptococcus suis was developed using the recombinant protein derived from the recombinant expressed epitopes region of Glutamate Dehydrogenase （rGDH） protein. The rGDH coated antigen was used at an optimal concentration of 6μg/mL and the rabbit anti-porcine IgG HRP conjugate was used at 1：2 000 dilution. S/P ≥0.210 was scored positive and S/P ≤0.166 negative. The assay was specific, sensitive and reproducible and could detect antibodies against all serotypes of Streptococcus suis.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第8期623-626,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家973计划(2006CB504401)
