摘要
目的探讨幽门螺杆菌耐药基因酶显色法芯片检测的寡核苷酸探针固定、探针浓度及杂交最佳条件。方法设计spacer为poly（dT）10的探针，与285bp的digoxigenin标记的靶序列杂交，探针浓度选择5～50μmol／L，杂交温度选择30～42℃，杂交时间选择0．5～1．5h。结果当spacer为poly（dT）10，探针浓度为15μmol／L，杂交温度为37℃，杂交时间为0．5h左右即可获得理想的杂交结果。结论通过优化杂交条件可以有效地提高杂交效率。
Objective To investigate the optimization of the hybridization conditions with immobilization of oligonucleotide probes,the concentration of probes for detecting clarithoromycin resistance gene of Helicobacter Pylori by coloration gen chip. Methods The probes with poly（dT）~0 as spacer were designed,and then hybridized with the digoxigenin labeled tar- get sequence of 285 bp at concentration of 5~50 μmol/L and temperature of 30~42 ℃ for 0. 5~1.5 h. Results The opti- mized condition of hybridization was that the hybridization at 37 ℃ for 0. 5 h and the concentration of probe was 15μmol/ L. Conclusion Elevated hybridization efficiency can he gained by optimization of hybridization conditions.
出处
《现代检验医学杂志》
CAS
2009年第4期65-67,共3页
Journal of Modern Laboratory Medicine
基金
基金项目：江苏省医学重点学科建设基金（XK200723）,江苏省卫生厅“科教兴卫”基金.
关键词
基因芯片
幽门螺杆菌
探针
优化
gene chip
Helicobacter pylori
probe
optimized
