摘要
目的通过检测南通地区强直性脊柱炎（AS）患者HLA—B27基因的亚型，来了解各亚型的分布及其与AS的相关性。方法用流式细胞术检测HLA—B27抗原，其基因亚型检测采用序列特异性引物聚合酶链式反应（PCR-SSR）技术。结果AS患者中HLA．B27阳性者占92．3％（48／52），正常人群中HLA—B27阳性为8．3％（30／360）。AS患者中发现四种亚型，分别是B2705占47．9％（23／48），B2704占43．8％（21／48），B2702占6．3％（3／48）和B2707占2．1％（1／48），正常人群中发现三种亚型，分别是B2705占50．0％（15／30），B2704占43．3％（13／30）和B2702占6．7％（2／30）。结论南通地区人群AS患者与HLA-27基因高度相关，其亚型有B2702，B2704，B2705和B2707，以B2705和B2704亚型为主，但各亚型分布在正常人群和AS患者中并没有明显差异。
Objective To investigate the distribution of HLA-B2？ alleles in healthy controls and in patients with ankylosing spondylitis （AS） in Nantong by detect the HLA-B27 subtypes. Methods The HLA-B27 antigen and its subtypes were de- tected by flow cytometry and PCR-SSP respectively. Results The positive ratio of HLA-B27 gene were 92.3% and 8.3% in AS patients and healthy controls respectively. Four B27 subtypes were identified in AS patients ：B2705 （47. 9%）,B2704 （43.8 %）, B2702 （6. 3 %） and B2707 （2. 1%）. And there were three B27 subtpyes in normal controls ： B2705 （50. 0% ）, B2704（43.3%） and B2702（6. 7%）. Conclusion AS is remarkably associated with HLA-B27 in Nantong area,and there are four subbypes：B2702,B2704,B2705 and B2707. The most prominent B27 alleles are B2705 and B2704,no significant differences are noticed in the frequencies of HLA-B27 alleles between patients and controls.
出处
《现代检验医学杂志》
CAS
2009年第4期135-136,共2页
Journal of Modern Laboratory Medicine
