摘要
目的同时测定鱼腥草注射液中萜品烯-4-醇、α-萜品烯醇和甲基正壬酮挥发性成分的含量。方法采用GC法,色谱柱为SE-54毛细管柱（30 mm×0.32 mm,0.25μm）,升温程序为：以2℃.min-1从80℃升至120℃,再以10℃.min-1升至200℃,保持5 min。以正十六烷为内标,选用C18固相萃取小柱对样品进行纯化浓集。结果萜品烯-4-醇0.03～0.30 mg.ml^-1线性关系良好（r=0.9996）,平均回收率为98.5%（RSD=1.0%）;α-萜品烯醇0.01～0.10 mg.ml^-1的线性关系良好（r=0.9998）,平均回收率为98.8%（RSD=0.2%）;甲基正壬酮0.01～0.10 mg.ml^-1的线性关系良好（r=0.9999）,平均回收率为99.9%（RSD=1.8%）。结论所建方法准确、简便、快速,可用于测定鱼腥草注射液中的3种挥发性成分。
OBJECTIVE To simultaneously determine the three main evanescent components in Yuxingcao injection.METHODS GC determination was performed on a SE-54 column（30 m×0.32 mm,0.25 μm）,and the programmed column temperature was elevated from 80℃ to 120℃ at 2℃·min-1,and then to 200℃ at 10℃·min-1,kept for 5 min.n-hexadecane was used as the internal standard（IS）,and the samples were purified by C18 SPE column.RESULTS A good linearity was obtained within the range of 0.03-0.30 mg·ml^-1（r=0.9996） and the average recovery of terpinen-4-ol was 98.5%（RSD=1.0%）.A good linearity was obtained within the range of 0.01-0.10 mg·ml^-1（r=0.9998） and the average recovery of α-terpinenol was 98.8 %（RSD=0.2%）.A good linearity was obtained within the range of 0.01-0.10 mg·ml^-1（r=0.9999） and the average recovery of methyl-n-nonylketone was 99.9 %（RSD=1.8%）.CONCLUSION This method is accurate,simple,rapid,and can be used to simultaneously determine the three main evanescent components in Yuxingcao injection.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第4期396-397,共2页
West China Journal of Pharmaceutical Sciences
