摘要
目的采用HPLC指纹图谱法测定熊胆胶囊中的胆汁酸类成分。方法采用C18色谱柱,以乙腈-甲醇-0.03 mol.L-1磷酸二氢钠溶液(磷酸调pH4.4)为流动相进行梯度洗脱;检测波长210 nm;柱温40℃。结果测定了10个熊胆胶囊样品中胆汁酸类成分的指纹图谱。结论为熊胆胶囊质量控制新模式的建立提供了依据。
OBJECTIVE To determine the fingerprint of bile acids of Xiongdan capsules by HPLC.METHODS The HPLC method for determination of fingerprint was：C18 column（200 mm×4.6 mm,5 μm）,mobile phase： acetonitrile-methanol-0.03 mol·L^-1NaH2PO4 solution（adjust pH to 4.4 by H3PO4）,detection wavelength：210 nm,Column temperature：40℃.RESULTS The fingerprints of 10 samples of Xiongdan Capsules had been determinated and compared with each other.CONCLUSION This study gives a good reference for developing a new mode of quality control of Xiongdan capsules.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第4期402-403,共2页
West China Journal of Pharmaceutical Sciences
