摘要
目的:建立仓鼠胰腺癌细胞株(pGHAM-1)移植性肝癌模型,并研究其生物学特性.方法:将pGHAM-1培养后配成4×1010/L,取0.5mL接种于仓鼠皮下,成瘤后,接种于仓鼠肝脏.分别于第20、30、40天用B超仪检测肝脏肿瘤大小.于接种后第40天处死动物,观察肿瘤的生长、转移及腹水量.解剖取出肝脏,用游标卡尺测量肿瘤大小,切开肝脏直接观察肿瘤,再进行HE染色的组织病理学检查,免疫组织化学检测血管内皮细胞生长因子(VEGF)及肿瘤转移相关蛋白(nm23-H1)的表达.结果:pGHAM-1种植性肝癌模型成功率达100%.B超仪检测仓鼠肝脏,第20、30、40天肿瘤体积分别为84.1±21.9mm3,413.7±208.4mm3,2187.3±1882.8mm3,与10d前者相比,有非常显著性差异(P<0.01);接种后第40天处死动物,解剖观察肿瘤,直接测量肿瘤体积为2948.0±2188mm3,其大小与第40天B超仪测量结果无显著性差异(P>0.05).部分仓鼠可见血性腹水;肝内未见肿瘤卫星结节;HE染色组织病理学检查为低分化胰腺癌;免疫组织化学检测结果显示VEGF及nm23-H1蛋白低表达.结论:pGHAM-1种植性肝癌模型建立方法简便,易复制,是理想的肝癌研究模型.
AIM: To establish a liver cancer model with an implanted pancreatic cancer cell line pGHAM-l on hamster and to analyze its biologic characteristics.
METHODS: Cell suspension of the cultured pancreatic cancer cell line pGHAM-1 of hamster in 4 × 10^10 cells/L was prepared and, each hamster was subcutaneously inoculated with 0.5 mL of the cell suspension. After the tumor was formed, hamster was then inoculated with the tumor cell. The size of the liver tumor was examined by B-ultrasound for each the hamster at the 20th, 30th, and 40th day after inoculation, respectively. At the 40th day after inoculation, the animals were sacrificed, and the growth of tumor, metastasis, ascites volume were observed, then the liver of the hamster was removed out and the tumor was measured with a venire caliper. The tumor was observed directly with dissection of the hamster liver. It was made that the histopathology examination of the slices was stained by HE and the immunohistochemical assay of vascular endothelial cell growth factor (VEGF) and tumor metastasis associated protein (nm23-H1).
RESULTS: Hamster liver cancer model induce by implanting pancreatic cancer cell pGHAM-1 was 100% successful. B-ultrasound examinations showed that the tumor volume was 84.1 ± 21.9 mm^3, 413.7 ± 208.4 mm^3, 2187.3 ± 1882.8 mm^3 at the 20th, 30th, and 40th day after inoculation respectively. There were very significant differences (P 〈 0.01) when compared later tumor volume with ten days before. Tumor volume measured directly was 2948.0 ± 2188 mm^3 when the animals were anatomized at the 40th day after inoculation, and there was no significant difference (P 〉 0.05) when compared it with that examined by B-ultrasound examinations at the 40th day. Bloody ascites was observed in some hamsters. The satellite nodules of the pancreatic cancer were not observed. The histopathological examination of the slices stained by HE indicated a poorly differentiated pancreatic cancer. The results of the immunohistochemistry assay showed a low expression of VEGF and nm23-H1 protein.
CONCLUSION: The method for creating a liver cancer model of hamster induced by implanting pGHAM-1 pancreatic cancer cell is simple, easy to replication, and is an ideal model for liver cancer study.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第18期1827-1831,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30760281
广西壮族自治区教育厅科研基金资助项目
No.200810MS141~~