摘要
为了研究cpt1基因与水稻根负向光性运动的关系,探讨IAA横向运输与水稻根负向光性运动的关系。检测了外源试剂CaCl2、EDTA以及IAA对水稻根的负向光性运动的效应,并采用RT-PCR的方法验证了不同外源试剂对cpt1基因表达的影响。结果表明,水稻根经光照24h后,与对照相比,1mgL-1的CaCl2和0.001mgL-1的IAA能明显促进水稻根负向光性的弯曲生长,1mgL-1的EDTA(CaCl2的抑制剂)明显抑制水根负向光性的弯曲生长;cpt1基因的表达受上述外源试剂诱导,并表现与水稻根的负向光性生长呈明显的正相关性,推测IAA的横向运输及其运输载体CPT1蛋白对水稻根的负向光性运动具重要作用。
With the purpose of studying the relationship between cpt1 gene and negative phototropism in rice roots, discussing the contribution of asymmetric distribution of IAA on negative phototropism in rice roots, CaCl2, EDTA and IAA were assayed for their effects on the negative phototropism in rice roots, as well as effects on the expression of cpt1 gene on the basis of reverse transcription-PCR. The result showed that, the negative phototropism in rice roots was improved by the treatment with 1 mg L-1 CaCl2 and 0.001 mg L-1 IAA in culture solution under light for 24 h but constrained by 1 mg L-1 EDTA. A similar effect was shown from the analysis of cpt1 gene expression, which suggested CPT1 protein could be induced by CaCl2 and IAA. The effects also showed a positive correlation between the expression of cpt1 and negative phototropism in rice roots. It could be supposed from present results that the asymmetric distribution of IAA is an important step for the negative phototropism process in rice roots, which probably is particularly associated with CPT1 protein as a carrier of IAA.
出处
《作物学报》
CAS
CSCD
北大核心
2009年第8期1558-1561,共4页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30871467)
江苏省高校研究生科技创新计划项目资助
