摘要
目的探讨补气利湿活血方对慢性肾衰竭(CRF)的作用机制。方法采用5/6肾脏切除法制备大鼠慢性肾衰竭模型,2周后随机分为模型组和补气利湿活血方高、低剂量组各10只,另设一组为假手术组(对正常大鼠行假手术)。补气利湿活血方低、高剂量组分别灌胃给予补气利湿活血方15,30g·kg-1·d-1,10mL·kg-1;假手术组和模型组灌胃给予等量纯化水。检测各组大鼠尿蛋白量、血清尿素氮(BUN)、肌苷(Cr)水平、肾脏病理。结果模型组大鼠尿蛋白、BUN与Cr显著增加。补气利湿活血方低剂量组BUN与Cr显著下降,补气利湿活血方高剂量组尿蛋白、BUN与Cr均显著下降,两给药组肾脏病变均减轻。结论补气利湿活血方能减慢CRF进程。
Objective To observe the effects of buqilishihuoxue fomula （the formula has the effects of invigorating vital energy ,eliminating clamp and promoting blood flow ）on the rats with chronic renal failure （CRF）. Methods CRF models were established by cutting 5/6 kidney of rats. Two weeks later, CRF rats were randomly divided into 3 groups ： the model group, high dose and low dose of buqilishihuoxue fomula groups, and the sham operated group was operated. Rats were all treated orally with 10 mL ·kg^-1·d^-1 of solution, pure water in sham and model groups. 15,30 g ·kg^-1 herbs in low dose and high dose groups . Levels of urine protein, serum creatinine （Cr）, blood urea nitrogen （BUN） were determined and the renal pathology were analyzed. Results It was shown that all the biochemistry markers in the model rats were significantly increased, but decreased in high dose of buqilishihuoxue fomula group. Compared with the model group, levels of SCr and BUN in the low dose of buqilishihuoxue fomula group were decreased obviously. The renal damages were attenuated in both buqilishihuoxue fomula groups. Conclusion Buqilishihuoxue fomula could step down the pathological proceeding of chronic renal failure.
出处
《医药导报》
CAS
2009年第8期974-977,共4页
Herald of Medicine
基金
江苏省中医药局项目(基金编号:2003017)
