摘要
为探讨硫酸镁对放射性脑损伤炎症反应的作用,用20Gy的电子线对SD大鼠进行全脑照射,在辐照后1、7和14d采用酶联免疫吸附法测定脑组织细胞间黏附分子(Intercellular surface adhesion molecule-1,ICAM-1)含量、分光光度法测定髓过氧化物酶(Myeloperoxidase,MPO)活性,苏木精-伊红(Hematoxylin-eosin,HE)染色法进行病理形态学观察。结果显示,放射性脑损伤大鼠脑组织中ICAM-1含量及MPO活性明显增加,引起放射性脑损伤继发性炎症反应;给予硫酸镁能显著降低放射性脑损伤大鼠的ICAM-1含量及MPO的活性,减轻炎性细胞浸润及脑组织损伤。早期给予硫酸镁可减轻放射性脑损伤大鼠脑组织的炎症效应,发挥细胞保护作用。
In order to ivestigate the effect of magnesium sulfate （MgSO4） on the inflammatory reaction, the whole brain of SD rats were irradiated with a dose of 20 Gy so as to establish radiation-induced brain injury model. The expression of intercellular surface adhesion molecule-1（ICAM-1） was measured serially with enzyme linked immunosorbnent assay （ELISA） and the activity of myeloperoxidase（MPO） in brain tissue was measured by the absorption spectrometry at 1, 7, 14 days after irradiation. The expression of ICAM- 1 and the activity of MPO in brain of rat irradiated with 20Gy increase markedly. MgSO4 can singificantly down-regulation the expression of ICAM-1 and the activity of MPO of the irradiated rats, and reduce tissue cell denaturalization and inflammatory cell infiltrate observed by use of light-microscopy. MgSO4 used in the early stage after irradiation could reduce the inflammatory reaction and protect brains from radiation injury.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2009年第4期234-238,共5页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金项目(30670638)资助
关键词
硫酸镁
电子线
大鼠
脑
炎症反应
ICAM-1
Magnesium sulfate, Electron, Rats, Brain, Inflammatory reaction, ICAM- 1
