摘要
目的观察脂多糖(LPS)刺激对小鼠腹腔巨噬细胞高迁移率族蛋白B1(HMGB1)表达的影响。方法取正常Swiss小鼠腹腔巨噬细胞,以LPS分别刺激4、8、12、18、24和36h后,RT-PCR法检测小鼠腹腔巨噬细胞HMGB1基因mRNA的转录水平,Western blot法检测HMGB1蛋白的表达水平,观察LPS刺激与HMGB1释放的时效关系。结果LPS刺激后4～18h,细胞内HMGB1基因mRNA的转录水平无明显变化,24h开始明显增强,刺激24h和36h与对照组相比,差异有统计学意义,HMGB1蛋白在LPS刺激后18h开始表达明显增加,并一直持续至36h。结论LPS可促进小鼠腹腔巨噬细胞HMGB1的表达,并具有时间依赖性。
Objective To observe the effect of stimulation with LPS on the expression of high mobility group box 1 （HMGB1） in murine peritoneal macrophages. Methods The peritoneal macrophages of normal mice were isolated and stimulated with LPS for 4, 8, 12, 18, 24 and 36 h respectively, then determined for transcription level of HMGB1 mRNA by RT-PCR and for expression level of HMGBI protein by Western blot. The time-response relationship between LPS stimulation and HMGB1 release was observed. Restilts The transcription level of HMGB1 mRNA showed no significant change 4 - 18 h, while increased significantly 24 h and showed significant difference with those of control 24 and 36 h after LPS stimulation. However, the expression level of HMGB1 protein increased significantly from 18 h until 36 h after LPS stimulation. Conclusion LPS showed a time-dependent promoting effect on the expression of HMGB1 in murine peritoneal macrophages.
出处
《中国生物制品学杂志》
CAS
CSCD
2009年第8期768-770,共3页
Chinese Journal of Biologicals
基金
吉林大学种子基金资助项目(701046)
关键词
脂多糖
巨噬细胞
高迁移率族蛋白B1
表达
Lipopolysaccharide （ LPS ）
Macrophage
High mobility group box 1 （ HMGB 1 ）
Expression
