摘要
目的:观察并比较银翘散主要药物(金银花、连翘、牛蒡子、荆芥、甘草)体外抑制流感病毒作用。方法:采用鸡胚培养法、血凝试验观察银翘散主要药物提取物不同时间点体外直接抑制流感病毒及对流感病毒感染鸡胚的预防和治疗作用。结果:金银花、连翘、牛蒡子、荆芥、甘草提取物均可直接抑制甲型流感病毒FM1株的活性,作用时间从1h可持续到24h,其中连翘30%醇提物抑制流感病毒增殖的作用最强;金银花、连翘、牛蒡子、荆芥、甘草提取物对流感病毒感染鸡胚均具有一定的保护和治疗作用,治疗作用优于预防作用,连翘对鸡胚的预防保护作用最强。结论:银翘散主要药物提取物均具有一定预防和治疗甲型流感病毒作用,其中以连翘作用最强。
Objective ：To observe and compare the effects of the main constituents of Lonicerae and Forsythiae Powder （flos lonicerae ,forsythia suspensa,fructus ,fineleaf schizonepeta herb,glycyrrhiza uralensis）inhibit influenza virus（IV） in vitro. Methods ： Cultivation in the chick embryo and hemagglutination test are applied to observe the extractives of the main constituents of Lonicerae and Forsythiae Powder inhibit IV in vitro at different times and the prevent and therapeutical effects on the viral infection chick embryo. Results ： The extractives of flos lonicerae, forsythia suspensa, fructus, fineleaf schizonepeta herb,glycyrrhiza uralensis could inhibit the activity of IVA FM1 strain, action time could continue from l h to 24h, and the 30% alcohol extractives of forsythia suspense is strongest in inhibit IV multiplication; The extractives of flos lonicerae, forsythia suspensa,fructus ,fineleaf schizonepeta herb, glycyrrhiza uralensis all have the protect and therapeutical effects on the viral infection chick embryo, and therapeutical effect is superior to the protect effect, the protect effect of forsythia suspense is strongest. Conclusion：The extractives of the main constituents of Lonicerae and Forsythiae Powder are all have the prevent and therapeutical effects on IVA, and the effect of forsythia suspense is strongest.
出处
《中华中医药学刊》
CAS
2009年第8期1634-1636,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(20080162004)
关键词
银翘散
甲型流感病毒
鸡胚培养法
血凝试验
Lonicerae and Forsythiae Powder
influenza virus A（IVA）
cultivation in the chick embryo
hemagglutination test
