摘要
通过骨组织形态计量学结合骨生物力学方法探讨酒精摄入对生长期小鼠骨代谢和力学性能的影响。清洁级昆明种小鼠30只随机分组。基础对照组实验开始时处死。正常对照组给予蒸馏水灌胃,酒精组给予50%乙醇4g·kg-1.d-1剂量灌胃共60d。处死后取胫骨上段、中段不脱钙骨切片进行骨组织形态计量学测量;取腰椎及股骨用于骨生物力学测定。与正常组比较,酒精组的松质骨小梁面积百分数(%Tb.Ar)及皮质骨面积百分率(%Ct.Ar)均降低(P均<0.05),两者动态骨形成率均降低(P<0.05)。腰椎抗压性能和股骨颈结构强度均明显下降(P<0.01)。小剂量酒精抑制生长期小鼠的骨形成,影响骨代谢并降低骨生物力学性能。
This study was aimed to detect the effects of alcohol on bone metabolism and biomechanical property of growing mice. Thirty KM mice were randomly divided into 3 groups, namely basal control group （mice were killed at the beginning）, normal control group （with distilled water given by gastrogavage）, and 50% （V/V） alcohol group （with alcohol given by gastrogavage at the dose of 4g .kg^-1 .d^-1 for 60 days）. All mice were killed and their proximal tibia and tibial diaphysis were processed by undecalcified sections and measured by bone histomorphometry. The biomechanical properties of lumbar vertebra and femur were tested. Compared with normal control, the index of trabecular bone area （% Tb. At） of proximal tibial metaphysis （PTM） and the static parameter of cortical bone（%Ct. At） both decreased obviously（P〈0.05） in alcohol group. Bone formation rate （BFR/TV） of trabeeular bone and cortical bone dropped also（P〈0. 05）. The maximal resistibility of lumbar vertebra and structural mechanical strength of proximal femoral neck both declined significantly （P〈0.01） in alcohol group. Low dose of alcohol inhibited the bone formation rate of growing mice , thus leading to a disorder of bone metabolism and a decrease in biomeehanical quality.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2009年第4期780-782,共3页
Journal of Biomedical Engineering
关键词
酒精
骨代谢
形态计量学
生物力学
小鼠
Alcohol
Bone metabolism
Histomorphometry
Biomechanics
Mice
