摘要
根据筛选到的与桃果实非酸/酸性状紧密连锁的3个AFLP标记特异片段序列信息,设计特异引物BFPA1/A2、BFPB1/B2和BFPC1/C2。3个特异引物在‘京玉’桃、‘美味’油桃及其正反交F1代69株群体中的PCR检测结果表明:引物BFPB1/B2在果实性状表现为酸的后代个体中扩增出分子量大小为158bp的特异条带,且根据特异片段AT-CTA199不同部位设计的引物都能稳定扩增,只是片段大小有所差异;特异扩增检测F1群体分离结果与原先AFLPAT/CTA标记完全一致,表明AFLP标记已成功转化为SCAR标记,该SCARBFPB1/B2标记与D基因的连锁距离为2.99cM。利用SCARBFPB1/B2标记对‘大久保’桃ב兴津’油桃的F2群体60株个体的果实非酸/酸性状进行检测,基因型与表型性状符合率为96.7%,并且表现为共显性标记。特异引物BFPA/A和BFPC/C的扩增多态性条带在亲本及后代中消失。
In this paper, specific primers of BFPA1/A2, BFPB1/B2, BFPC1/C2 were designed respectively according to the sequence of three specific fragments from AFLP markers which linked to non-acid/acid trait of peach. A 158 bp polymorphic band was amplified from the DNA of 69 F1 population of the inti＇a,specific cross between cultivars‘Jingyu＇ and‘Flavortop＇ with the primer pair BFPB1/B2. Furthermore, the steady and specific amplification can be got with the primers designed in different location of the AFLP fragment AT-CTA199. The SCAR marker had the same segregation pattern as the original AFLPAT/CTA marker with a linkage distance of 2.99 cM and the AFLP marker was converted into SCAR marker successfully. The SCARBFPB1/B2 marker was tested in 60 F2 progeny of‘ubo＇ and ＇ Xingjin＇ nectarine, and the results showed that the accordance ratio of genotype and phenotype was 96.7%. The SCAR marker was a co-dominant marker and could distinguish the heterozygous and homozygous. Polymorphism disappeared in parents and progeny with specific primers BFPA1/A2 and BFPC1/C2.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第8期1120-1126,共7页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2006AA100108-2-1)
