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氯胺酮致小鼠精神分裂样症状及其机制探讨 被引量:2

Study on the Schizoid Symptoms of the Mice Induced by Ketamine and Its Mechanism
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摘要 目的观察氯胺酮致小鼠精神分裂样症状,血清肌酸激酶(CK)、一氧化氮(NO)的改变,及对额叶MEK1 mRNA、ERK2 mRNA表达的影响。方法将40只昆明小鼠随机分为生理盐水组和氯胺酮小、中、大剂量4组。腹腔注射7d后观测行为学改变,用紫外分光光度法测定血清CK和NO水平,用RT-PCR方法检测额叶MEK1、ERK2的表达。结果与生理盐水组相比,给药组小鼠在行为学指标上有明显改变,以大、中剂量组显著,差异有高度统计学意义(P<0.01);各给药组小鼠血清CK活性均有不同程度增高,但仅大剂量组的差异有高度统计学意义(P<0.01),血清NO含量均显著增高,差异均有高度统计学意义(P<0.01);额叶MEK1的表达,中剂量组和大剂量组均明显下调,ERK2的表达在大剂量组明显下调,差异均有统计学意义(P<0.05或<0.01)。结论氯胺酮增加了小鼠血清CK活性和NO含量,抑制MEK1-ERK2信号转导通路的激活,使其产生精神分裂样症状,这可能也是精神分裂症的发病机制之一。 Objective To observe the schizoid symptoms of mice induced by ketamine,the changes of CK and NO in serum, and the influences to expression of MEK1 and ERK2 mRNA in frontal lobe. Methods Forty Kunming mice were divided into 4 groups randomly the control group of physiological saline, ketamine groups including small dose group, medium dose group and large dose group, all mice were administrated by intraperitoneal injection and the changes of their behavior were observed after 7 days, CK and NO in serum were determined by ultraviolet spectrophotometer technique, the expression of MEK1 and ERK2mRNA in frontal lobe,were observed by RT-PCR technique. Results Compared to the control group,obvious changes were observed in mice's behavior in every ketamine group,especially in the medium dose group and the large dose group (P〈0.01). The activity of CK in serum was increased in every ketamine group, and significant difference in large dose group (P〈0.01), the content of NO in serum was increased significantly (P〈0.01). In frontal lobe, the expression of MEK1 mRNA was decreased obviously in ketamine me-dium dose group and large dose group,the expression of ERK2 mRNA was decreased obviously in ketamine large dose group(P〈0.05 or 〈0.01). Conclusion Ketamine increase the activity of CK and content of NO in serum, inhibited the activation of MEK1- ERK2 signaling transduction pathway, and then induce the schizoid symptoms of themice,that may be a pathogenesy of schizophrenia.
出处 《苏州大学学报(医学版)》 CAS 北大核心 2009年第3期439-442,共4页 Suzhou University Journal of Medical Science
基金 香港保健协会资助项目(20060909-10HK)
关键词 氯胺酮 精神分裂样症状 MEK1 MRNA ERK2mRNA ketamine schizoid symptoms MEK1 mRNA ERK2 mRNA
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  • 1张德林,庄小凤,裘卫东,蒋豪,薛张纲.数量化脑电参数对异丙酚、咪唑安定或氯胺酮麻醉病人镇静深度监测的准确性[J].中华麻醉学杂志,2005,25(3):179-182. 被引量:11
  • 2王贤裕,田玉科,安珂,金小高.氯胺酮对术后大鼠海马NMDAR亚单位蛋白表达的影响[J].华中科技大学学报(医学版),2005,34(3):372-374. 被引量:12
  • 3周晓林,张进,王丹,戴体俊.东莨菪碱与氯胺酮合用对小鼠学习记忆的影响[J].徐州医学院学报,2006,26(2):137-139. 被引量:8
  • 4陈丽萍,马光瑜.慢性酒精作用对阿片系统的影响和学习与记忆的关系[J].国际精神病学杂志,2006,33(2):123-126. 被引量:8
  • 5Enomoto T, Floresco SB. Disruptions in spatial work- ing memory, but not short-term memory, induced by repeated ketamine exposure[J]. Prog Neuropsychophar- macol Biol Psychiatry, 2009,33 (4) :668-675.
  • 6Vasconcelos SM, Sales GT, Lima NM, et al. Deter- mination of amino acid levels in the rat striatum, af- ter administration of ethanol alone and associated with ketamine, a glutamatergic antagonist[J]. Neurosci Lett, 2008,444 ( 1 ) : 48-51.
  • 7Pitsikas N, Boultadakis A. Pre-training administration of anesthetic ketamine differentially affects rats' spa- tial and non-spatial recognition memory[J]. Neurophar-macology, 2009,57 ( 1 ) : 1-7.
  • 8Goulart BK, de Lima MN, de Farias CB, et al. Ke- tamine impairs recognition memory consolidation and prevents learning-induced increase in hippocampal brain-derived neurotrophic factor levels[J]. Neuroscience, 2010, 167(4) :969-973.
  • 9Morgan C J, Riccelli M, Maitland CH, eta!. Long- term effects of ketamine: evidence for a persisting impairment of source memory in recreational users[J]. Drug Alcohol Depend, 2004,75 (3) : 301-308.
  • 10Wang JH, Fu Y, Wilson FA, et al. Ketamine affects memory consolidation: differential effects in T-maze and passive avoidance paradigms in mice[J]. Neuro- science, 2006,140(3 ) : 993-1002.

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