摘要
建立了定量分析ｍＲＮＡ表达的ＲＮａｓｅ保护法，并用于鲤鱼ＩＧＦ－Ｉ的研究．结果表明鲤鱼ＩＧＦ－ＩｍＲＮＡ在肝组织的丰度为每微克ＤＮＡ含５０．４５×１０－１７ｍｏｌ，在其他组织有少量ＩＧＦ－ＩｍＲＮＡ表达．
Insulinlike growth factorI interests many investigators because of its importance in mediating most growth effects of growth hormone. To study carp IGFI expression, we established the quantitative method for detecting mRNA, RNase Protection Assay (RPA). This technique is rapid and requires minimal sample manipulation, allowing the analysis of hundreds of samples simultaneously. From the study on common carp, the hepatic IGFI mRNA expression was shown highest, 5445×10-17 mol/μg DNA. In other tissues, low amount of IGFI mRNA was also expressed.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期121-124,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
中山大学基础学科前沿课题基金
