摘要
目的:研究切口痛大鼠脊髓背角磷酸化p38有丝分裂原活化蛋白激酶(p-p38MAPK)表达的变化和鞘内注射p38MAPK特异性抑制剂SB203580对切口痛大鼠疼痛的影响。方法:雄性SD大鼠随机分为假手术组(SH组)、切口痛组(IP组)、药物组和DMSO组。按Yaksh法施行鞘内置管。按Brennan法建立大鼠切口疼痛模型。采用Hargreaves法(热痛觉过敏)测定热刺激缩足反射潜伏期(TWL)。应用免疫组织化学法检测脊髓背角p-p38MAPK表达的变化。结果:与SH组相对应的时间点和术前值比较,IP组和DMSO组大鼠在术后2 h、3 h、6h、1d、2 d和3 d的TWL均明显缩短(P<0.05或P<0.01),但IP组和DMSO组各相对应的时间点比较无明显差异;与IP组和DMSO组相对应的时间点比较,药物组大鼠在术后2 h、3 h、6 h、1 d和2 d的TWL均明显延长(P<0.05或P<0.01)。与SH组比较,IP组和DMSO组p-p38MAPK阳性细胞数增加(P<0.01);与IP组和DMSO组比较,药物组p-p38MAPK阳性细胞数降低(P<0.01或0.05),IP组和DMSO组间差异无统计学意义(P>0.05)。IP组和DMSO组p-p38MAPK阳性细胞数在术后6h开始增加,1d达到高峰,然后逐渐下降,5d后逐渐恢复。结论:脊髓背角p38MAPK活化参与切口痛的形成与发展。
Objective：To investigate the change of phosphorylated p38 mitogen-activated protein kinase （p-p38MAPK） in the spinal dorsal horn, and the effects of intrathecal administration of p38MAPK specific inhibitor SB203580 on the behavior of pain in a rat model of incisional pain. Methods： Male Spra- gue-Dawley rats were randomly divided into sham group, incisional pain group, intrathecal drug group and intrathecal DMSO group. Catheter was inserted into the intrathecal space according to Yaksh~ method in all rats. The incisional pain model was adopted as described by Brennan et al. Thermal withdrawal la- tency（TWL） was measured using radiant heat method. Using immunohistochemistry method, the expres- sions of p-p38MAPK in the spinal dorsal horn following paw incision surgery were investigated. Results： Compared to sham group and baseline of pre-incision, TWL ipsilateral to the incision in 2 h, 3 h, 6h, ld,2d and 3d post-incision decreased significantly in the IP group and DMSO group （P 〈0.05 or P 〈0. 01 ） ; but compared to IP group, the change of TWL in corresponding point of time post-incision in DMSO group had no significant change;Compared to TWL in corresponding point of time after incision in IP group and DMSO group, TWL ipsilateral to the incision in 2 h, 3 h, 6h, 1 d and 2d of post-incision increased significantly in drug group （ P 〈 0.05 or P 〈 0.0I ）. Compared to sham group, the number of positive cells of p-p38MAPK in the spinal dorsal horn ipsilateral to the paw surgery increased significantly in IP group and DMSO group （P 〈0.01 ） ;Compared to IP group and DMSO group, the number of positive cells of p-p38MAPK in the spinal dorsal horn ipsilateral to the paw surgery decreased significantly in drug group（P 〈0.05 or P 〈 0.01 ）;The number of positive cells between IP group and DMSO group had no significant change （P 〉 0.05 ）. The number of positive cells of p-p38 MAPK in IP group and DMSO group began to increase from 6h, reached peak on 1 d, but decreased to baseline of pre-incision after 5d of post- incision. Conclusions： p38MAPK activation in spinal dorsal horn plays important role in the formation and development of incisional pain in rats.
出处
《中国疼痛医学杂志》
CAS
CSCD
2009年第4期226-229,共4页
Chinese Journal of Pain Medicine
基金
江苏省重点实验室开放课题(KJS06002)
