摘要
目的:建立HPLC法同时测定血府逐瘀颗粒中芍药苷、阿魏酸、柚皮苷及甘草酸的含量。方法:采用SepaxC18(4.6mm×250mm,5μm)色谱柱;以0.1%磷酸水溶液(A)-70%乙腈(B)为流动相进行梯度洗脱(0～30min,24%B→40%B;30～50min,40%B→100%B;51～60min,24%B),流速1.0mL·min-1;检测波长:0～12min时230nm(芍药苷),12.1～15.5min时320nm(阿魏酸),15.6～30min时283nm(柚皮苷),30.1～60min时250nm(甘草酸);柱温40℃。结果:芍药苷、阿魏酸、柚皮苷及甘草酸的线性范围分别为0.05～0.65,0.01～0.13,0.05～0.65,0.02～0.28μg(r≥0.9990);平均回收率(n=6)分别为101.9%(RSD=2.0%),104.3%(RSD=2.4%),104.4%(RSD=1.8%),102.9%(RSD=2.1%)。结论:所建立的HPLC法可同时测定血府逐瘀颗粒中芍药苷、阿魏酸、柚皮苷及甘草酸的含量。
Objective：To develop an HPLC method for determination of paeoniflorin,ferulaic acid,naringin and glycyrrhizic acid in Xuefu Zhuyu granules.Methods：The analysis was carried out on an Sepax C18（250 mm×4.6 mm,5 μm） column;The mobile phase was composed of 0.1% phosphoric acid aqueous（A） and 70% acetonitrile（B） with gradient elution（0-30 min,24% B→40% B;30-50 min,40% B→100% B;51-60 min,24% B） and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 230 nm for paeoniflorin（0-12 min）,320 nm for ferulaic acid（12.1-15.5 min） and 283 nm for naringin（15.6-30 min） and 250 nm for glycyrrhizic acid（30.1-60 min）;The column temperature was 40 ℃.Result：The linear ranges of paeoniflorin,ferulaic acid,naringin and glycyrrhizic acid were 0.05-0.65μg,0.01-0.13μg,0.05-0.65μg,0.02-0.28μg（r≥0.9990,respectively）;The average recoveries were 101.9%, 104.3%,104.4% and 102.9% （RSD〈2.5%,n=6,respectively）.Conclution： This method is simple and can be used to determine the above 4 components with satisfactory accuracy and repeatability.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第8期1253-1255,共3页
Chinese Journal of Pharmaceutical Analysis
