摘要
试验旨在探讨试验性感染绵羊肺腺瘤病毒内蒙株(jaagsiekte sheep retrovirus,JSRV-NM)对羊的淋巴细胞亚群、淋巴细胞凋亡、血气指标的影响。用含JSRV-NM株的病毒悬液1 mL气管内接种1日龄羔羊,对人工感染羊进行了临床症状观察、血液学检查、病理生理学的系统病理学研究。在不同时段用流式细胞仪检测外周血中淋巴细胞及其亚群的动态变化及淋巴细胞凋亡情况。同时,对血液做血气分析和血清酶的检测。人工感染羔羊均出现了一些轻微的绵羊肺腺瘤病(sheep pul-monary adenomatosis,SPA)临床症状。SPA攻毒组感染JSRV-NM株后前60 d,外周血中CD4+、CD8+T细胞保持较高水平;到感染后90 d呈现下降趋势。对淋巴细胞凋亡检测的结果显示,在感染JSRV-NM株前60 d,凋亡细胞都保持较高水平;而感染第90天后,晚期细胞凋亡显著低于对照组。血气分析结果显示,阴离子隙(AnGap)、细胞外液碱剩余(BE(ecf))、氯离子浓度(Cl-)、氧分压p(O2)、实际碳酸氢根(HCO3act)、标准碳酸氢根(HCO3std)、二氧化碳总量(TCO2)、氧饱和度(O2SAT)、氧含量(O2CT)9项在不同时期与对照组存在显著差异。接毒羔羊尚未发生SPA的典型症状,但试验羔羊的病情已经出现,处于SPA疾病的早期表现;病羊在感染JSRV-NM病毒株后,在一定时期内,血液中CD4+T淋巴细胞和CD8+T淋巴细胞的变化是SPA病羊在疾病过程中细胞免疫和淋巴细胞受损的重要表现之一;外周血淋巴细胞会呈现不同程度的凋亡,JSRV-NM株诱导机体免疫细胞凋亡是一种重要的临床表现;本试验结果初步证明,血气分析方法在临床诊断绵羊肺腺瘤病时有一定的参考价值。
To examine the effects on the lymphocytes subsets, lymphocytes apoptosis, blood-gas of sheep experimentally infected with JSRV-NM strain. Five newborn lambs within 24 hours after their birth were inoculated intratracheally with cell-free filtration of pulmonary tumour tissue containing JSRV-NM strain from natural cases, 1.0 mL per lamb, respectively. At 30, 60, 90 and 120 d post-infection, used the flow cytometry to examined lymphocytes apoptosis number, CD4^＋ T and CD8^＋ T lymphocytes, analysis of blood-gas and serum enzymes. At 30 and 60 d post-infection, CD4^＋ T, CD8^＋ T lymphocytes and lymphocytes apoptosis were significantly higher than that of control, and decrease at 90 d post-infection except for the early lymphocytes apoptosis, AnGap, BE（eef）, CI^-, p（O2）, HCO3act, HCO3std, TCO2, O2SAT, O2CT showed significant difference between the experimental group and the control group. The typical symptoms of SPA have not appeared, and the disease was in the initial stage. The changes of CD4^＋ T, CD8^＋ T lymphocytes in peripheral blood of sheep experimentally infected with JSRV-NM strain demonstrated which may be the result of cell immunological reaction and the lymphocytes injury.Periphery lymphocyte apoptosis in sheep experimentally infected with JSRV-NM strain was found, lymphocyte apoptosis may be one of the most important clinical symptoms. The detection of blood-gas and the serum enzymes of sheep experimentally infected with JSRV NM strain was helpful for diagnosis of SPA.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第8期172-178,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(30660136)
关键词
绵羊肺腺瘤病
绵羊肺腺瘤病病毒
人工感染
血气分析
流式细胞术
sheep pulmonary adenomatosis
sheep pulmonary adenomatosis virus
experimentally induced
analysis of blood-gas
flow cytometry method
