摘要
目的:研究药用植物朱砂根组培快繁技术,为其产业化生产提供科学依据。方法:考察不同基本培养基、植物激素、添加物等对腋芽诱导、增殖及植株再生的影响。结果:以朱砂根带芽茎段为外植体,初代培养腋芽诱导的最佳培养基为MS+6-BA 0.5 mg.L-1+NAA 0.1 mg.L-1。腋芽增殖的最佳培养基配方为MS+6-BA 2.0 mg.L-1+NAA 0.1 mg.L-1+KT 0.5 mg.L-1。生根培养基为1/2MS+IBA 0.2 mg.L-1;添加0.2%的活性炭可明显促进根的生长,提高生根率、生根数。最有利于朱砂根无菌苗移栽存活的基质类型为河沙-珍珠岩-蛭石(1∶1∶1),或蛭石-珍珠岩(1∶1),成活率在80%以上。结论:通过腋芽增殖快繁育苗技术可获得完整植株,达到快速繁殖的目的。
Objective： Our research studied the fast-breeding technology of Ardisia crenata sims by using tissue culture and provided the scientific foundation for industry production. Method： The effects of axillary buds and plant regeneration of different basic medium, hormones and additives on induction and multiplication were studied. Result： The best culture medium for the induction of axillary buds, which took the stems of A. crenate were as explants, was MS ＋ 6-BA 0.5 mg·L^-1 ＋ NAA 0. 1 mg·L^-1 , and the best medium for multiplication was MS ＋ 6-BA 2.0 mg·L^-1 ＋ NAA 0. 1 mg·L^-1 ＋ KT 0. 5 mg·L^-1, the best medium for roots generation was 1/2MS ＋ IBA 0. 2 mg·L^-1. We also found that the roots＇generation, roots rate and mean number of roots can be promoted by adding 0. 2% Ac, and the most suitable ground substance was river sand-perlite-vermiculite （ 1 ： 1 ： 1 ） or perlite-vermiculite （ 1 ： 1 ）. With axillary buds and plant regeneration methode, more than 80% A. crenata sims could be regenerated integratedly. Conclusion： A. crenata sims can be regenerated integratedly and breeded fast by using axillary bud proliferation technology.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第16期2043-2046,共4页
China Journal of Chinese Materia Medica
基金
四川省教育厅攻关项目(2006A016)
关键词
朱砂根
组织培养
植株再生
Ardisia crenata
tissue culture
plant regeneration
