摘要
中功率密度He-Ne激光照射DNA固体纤维后,在很短的照射时间,激光对DNA磷酸骨架结构和氢键的损伤就达到了最大值,这时不仅有磷酸二酯键的断裂,氢键也发生了断裂;随着照射时间的延长,激光对DNA的损伤程度先是减小,然后再加剧。激光对碱基的影响,先是使碱基谱线强烈减色,随着照射时间的延长,减色效应逐渐减小,然后变为较强的增色效应,再变为减色效应,这也许是激光在损伤碱基后又破坏碱基-碱基间的垂直堆积状况,造成碱基谱线的拉曼增色效应。
When the DNA solid fiber was irradiated by middle - power density He - Ne laser, the damage of phosphoric acid skeleton structure and of hydrogen bonds has got to the maximum in the very short irradiation time. By now not only had the di - phosphate ester key was broken, the hydrogen bond has also been broken. Along with irradiation time＇s extension, the DNA damage induced by the laser is first to reduce, then to aggravate again. First the effect of laser irradiation on the bases was intense Raman hypochromicity of the base peaks. Second Raman hypochromicity of the base peaks weakened gradually with laser irradiation time elongation;then the stronger Ram,an hyperchromicity of the base peaks emerged. The base peaks became weak again when the laser irradiation continued. The reasons for these phenomena may be that laser destroyed the stacking structure of base - base which perpendicular with DNA after it damaged bases, so resulted in base peaks weakening first and strengthening subsequently.
出处
《激光杂志》
CAS
CSCD
北大核心
2009年第4期73-74,共2页
Laser Journal
基金
贵州省科技厅基金资助项目(20062003)
