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红霉素抑制A549细胞中鼻病毒14介导的白介素8和MUC5AC的分泌 被引量：1

Inhibition of erythromycin on rhinovirus-14-induced interleukin-8 and MUC5AC secretion in A549 cells
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摘要 目的探讨红霉素是否对鼻病毒14（RV14）介导的细胞因子产生和气道黏液高分泌有抑制作用。方法EM预处理肺泡Ⅱ型上皮细胞A5493h后，用RV14刺激细胞，然后采用酶联免疫吸附试验法检测细胞培养上清中细胞因子自介素8和细胞裂解液中黏液蛋白MuC5AC的浓度，并用Western blot检测磷酸化p44／42MAPK信号分子变化情况。结果红霉素明显抑制RV14介导的白介素8和MuC5Ac的产生和分泌，并且对RV14介导的p44／42MAPK的激活也有抑制作用。结论红霉素可有效抑制RV14介导的细胞因子和黏液蛋白的产生和分泌，并且这种抑制作用可能是通过阻断p44／42MAPK信号分子的激活来实现的。 Objective To examine the effect of erythromycin on rhinovirus-14 （RV14）-induced cytokines and airway mucin hypersecretion. Methods A549 cells were pre-incubated with medium containing 10umol/L erythromycin for three hours before RV14 infection. Immunoreactive interleukin-8 （IL-8） and MUC5AC were quantitated using dual antibody ELISA kits according to the manufacturer＇s protocol. And activated p44/42MAPK was tested by Western blot. Results Erythromycin blocked the hypersecretion of IL-8 and MUCSAC protein, and the activation of p44/42MAPK induced by RV14 in the A549 cells. Conclusions Erythromycin may inhibit RV14-induced IL-8 and MUCSAC hypersecretion by blocking the p44/42MAPK pathway or its upstream regulators.
作者 吴超民 罗志兵 沈策
机构地区 上海交通大学附属第六人民医院呼吸内科
出处 《国际呼吸杂志》 2009年第16期970-973,共4页 International Journal of Respiration
关键词 红霉素 鼻病毒 A549细胞 MUC5AC 白介素8 Erythromycin Rhinovirus A549 cell MUC5 AC Interleukin-8
分类号 R [医药卫生]
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参考文献19

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共引文献9

同被引文献11

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国际呼吸杂志
2009年 第16期

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