摘要
在血液与生物材料的相互作用中，血浆蛋白特别是纤维蛋白原（Ｆｉｂｒｉｎｏｇｅｎ，ＦＧ）的吸附起着重要的作用。本文利用酶联免疫吸附检测手段（ＥＬＩＳＡ），测定了两种鼠抗人ＦＧ的单克隆抗体Ｍ１和Ｒ１同吸附在材料表面的ＦＧ结合的亲合力（Ｋａ）。由于两种抗体与ＦＧ结合的位点不同，其Ｋａ值随温度和放置时间的变化呈现不同的变化规律，从而推测材料表面吸附的ＦＧ的构象变化。
Protein adsorption, especially, fibrinogen (FG) adsorption played a very important part in the action of blood with biomaterials. In this paper, the affinity Ka of the two mouse moloclonal anti-human FG antibodies M1 and R1 and adsorbed FG to biomaterials was determined by Enzyme Linked Immunosorbent Assay (ELISA). Because the two antibodies were baund with FG in different sites, based on the difference of the effect of temperature and residence time on Ka, the conformation changes of adsorbed to FG in the surface of biomaterials could be deduced.
