摘要
将0．4，0．8，1．6，2．9kbGBSS基因的5＇侧翼区与GUS基因融合，构建了双元表达载体．0．8kbGBSS－GUS通过基因枪转化法在块茎切片中获得了瞬间表达．已将上述构建体通过农杆菌转化法导入了马铃薯．X－Gluc染色及PCR结果均证实已获得转基因再生植株．在离体诱导块茎中，2．9，1．6，0．4kbGBSS启动子驱动的GUS活性均明显高于茎段，高出1～15倍；1．6，2．9kbGBSS启动子驱动的GUS活性则大大高于0．8，0．4kbGBSS启动子驱动的GUS活性；其中2．9kb的GBSS启动子显示最强烈的块茎表达专一性．
Binary vectors were constructed through fusing 0. 4,0. 8, 1. 6 and 2. 9 kb 5'flanking regions of GBSS gene with the GUS gene. Transient GUS expressions were observed on tuber slices bombarded with the 0. 8 kb GBSS-GUS construct. These constructs were then introduced into potato via Agrobacterium-mediated transformation. Integration of the foreign DNA into the genome was tentatively demonstrated using X-Glue staining and the PCR. Expression levels of the GUS gene under the control of 2. 9, 1. 6 and 0. 4 kb GBSS promoters in microtubers were 1 to 15 times higher than those in stems, with the 2. 9 kb being the most tuber specific. GUS activities were much stronger when the 2. 9 and 1. 6 kb GBSS promoters were used.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第4期559-563,共5页
Journal of Fudan University：Natural Science
基金
国家科委八六三高科技项目
国家自然科学基金
关键词
马铃薯
GBSS
GUS
块茎专一表达
基因表达
启动子
Solanum tuberousm L
granule-bound starch synthase gene
promoter
betaglucuronidase
tuber-specific expression
