摘要
利用SSR标记研究了40个糯玉米自交系的遗传多样性。用32对扩增带型稳定的SSR引物从供试材料中检测出152个等位基因变异,每对引物检测等位基因2～9个,平均4.75个。SSR引物的PIC介于0.303～0.862,平均多态性信息量为0.632。利用UPGMA聚类分系法将供试自交系划分为5类,该划分结果与根据地理来源、种质系谱的分类结果基本一致。SSR分子标记辅助的自交系改良是糯玉米品种改良的重要途径。
The genetic diversity of 40 waxy corn inbred lines were studied by simple sequence repeats （SSR） markers. 32 SSR primers giving stable amplified band pattern detected 152 alleles among the lines tested. The average number of alleles per SSR locus were 4.75 with a range from 2 - 9. The value of polymorphism information content （PIC） for each SSR locus varied from 0.303 to 0.862 with average of 0.632. The 40 inbred lines were divided into 5 groups by UPGMA method based on SSR fingerprinting. The clustered results were similar to that based on geographical resource and germplasmic genealogy. SSR marker could be an accurate and reliable method to study diversity of waxy corn inbred line. SSR marker assisted genetic improvement of waxy corn inbred lines would be an important way to breed new waxy corn cultivars.
出处
《玉米科学》
CAS
CSCD
北大核心
2009年第4期32-35,共4页
Journal of Maize Sciences
基金
陕西省13115科技创新重大专项(2007ZDG-03)
