摘要
目的:探讨白介素28B(IL-28B)在肺纤维化发病中的表达及意义。方法:健康昆明种小鼠48只,随机分成实验组和对照组,每组24只。实验组:气管内一次性注入生理盐水稀释的博来霉素(5mg/kg);对照组:气管内注入生理盐水0.06～0.07mL。分别于给药后第7、14、21、28天,每组各处死小鼠6只。无菌操作下取出小鼠肺组织,病理学方法观察各阶段肺内炎症及纤维化程度;检测肺组织内羟脯氨酸的含量,Westernblot检测肺内IL-28B的蛋白表达。结果:实验组肺组织病理切片按时间顺序呈现由肺泡炎至纤维化动态改变,实验组肺泡炎和纤维化程度均高于对照组(均P<0.01)。实验组肺组织内羟脯氨酸在第14～28天明显高于对照组(P<0.01)。实验组第7、14、21天的IL-28B的蛋白表达也高于对照组(P<0.05或P<0.01);IL-28B表达高峰在第14天,此后逐渐下降,第28天时表达水平与对照组差异无统计学意义(P>0.05)。结论:IL-28B在致肺纤维化损伤中发挥重要作用,检测IL-28B的水平对肺纤维化的早期诊断有帮助。
Objective ：To determine the expressions of interleukin（IL）-28B in lung tissues of mice with pulmonary fibrosis （PF） induced by bleomycin and the effect of the eytokine on PF thereof. Methods： Forty eight male mice were randomly divided into two groups ： experimental group（ n = 24） and control group （ n = 24）. The mice were treated with bleomycin solutions（5 mg/ kg） and saline （0.06-0.07 mL）by intratracheal instillation respectively. On day 7, 14, 21 and 28 after administration, 6 mice of each group were sacrificed and the lung tissues were harvested. The pulmonary inflammation and fibrosis were observed by pathological method in mice of different groups. Hydroxyproline concentrations were examined. The method of Western blot was adopted to detect the protein expression of IL-28B in lung tissue of mice. Results：In experimental group, dynamic changes from alveolitis to pulmonary fibrosis could be observed in the slices by pathological method. Hydroxyproline concentrations were higher in experimental group than those in control group on day 14,21 and 28. The protein expressions of IL-28B were higher in lung tissues of experimental group than those in control group during early periods （P 〈 0.05）, and peaked on the 14th day. On the 28th day its level was similar in experimental group to that of the control group. Conclusion：IL-28B protein may contribute to the pathogenesis of pulmonary fibrosis. Detection of IL-28B is helpful to early diagnosis of pulmonary fibrosis. Key words interleukins interferons pulmonary fibrosis hydroxyproline alveolitis, extrinsic allergic mice, inbred strains
出处
《天津医药》
CAS
北大核心
2009年第9期762-764,I0002,共4页
Tianjin Medical Journal
基金
卫生部科研项目(项目编号:wkj2006-2-026)
江苏省科技厅发展计划项目(项目编号:BS2001020)
