摘要
背景:星形胶质细胞是中枢神经系统的主要成分之一,具有对各种损伤产生强烈反应的特性,脑出血后星形胶质细胞大量表达、活性增强,这种由常态转变为反应性状态的星形胶质细胞的病理生理学意义是目前研究热点。目的:探讨重组人粒细胞集落刺激因子对脑出血后星形胶质细胞表达的影响。设计、时间及地点:随机对照动物实验,于2006-03/11在泸州医学院中心实验室完成。材料:清洁级健康雄性SD大鼠50只,随机分为3组:假手术组10只、模型组20只、实验组20只。重组人粒细胞集落刺激因子为深圳新鹏生物工程有限公司产品。方法:模型组、实验组利用鼠脑立体定向仪、采用断尾取自体血法制备脑出血动物模型,假手术组用等量生理盐水代替自体血,余干预措施与模型组一致。造模1h后,实验组大鼠腹腔注射重组人粒细胞集落刺激因子60μg/kg,余2组未注射任何物质。主要观察指标:分别于干预后6h,24h,48h,72h,7d采用免疫组织化学ABC法检测胶质纤维酸性蛋白阳性细胞的表达。结果:假手术组未见胶质纤维酸性蛋白阳性细胞的表达。模型组干预6h即有少量胶质纤维酸性蛋白阳性细胞表达,48h后开始增多,至72h胶质纤维酸性蛋白阳性细胞数量达高峰(P<0.05),7d后仍有大量胶质纤维酸性蛋白阳性细胞表达,但较72h时有所减少。实验组干预6h时胶质纤维酸性蛋白阳性细胞的表达与模型组相似,干预24h,48h,72h,7d时胶质纤维酸性蛋白阳性细胞较模型组明显减少(P<0.05),细胞变形程度减轻。结论:重组人粒细胞集落刺激因子能够抑制脑出血后星形胶质细胞的过度活化,可能对损伤脑组织重建和功能恢复起重要作用。
BACKGROUND： Astrocytes serve as a major component of central nervous system, which reacted actively to various damages. The astrocytes were strongly expressed with enhanced activity after intracerebral hemorrhage. The pathophysiological significance of this change is presently the research hotspot. OBJECTIVE： To investigate the effects of recombinant human granulocyte colony-stimulating factor （rhG-CSF） on the astrocytes expression after intracerebral hemorrhage. DESIGN, TIME AND SETTING： A randomized control experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006. MATERIALS： Fifty healthy, male, SD rats were randomly divided into sham operation （n=10）, model （n=20） and experimental （n=20） groups. The rhG-CSF was purchased from Xinpeng Bioengineering Co., Ltd. METHODS： According to rat brain stereotaxic atlas, intracerebral hemorrhage model was prepared by the method of cutting off tail to obtain blood. The blood was replaced with physiological saline in the sham operation group. Totally 60μg/kg rhG-CSF was administered by intraperitoneal injection at 1 hour after model preparation. There was no injection in the other two groups. MAIN OUTCOME MEASURES： The expression of glial fibrillary acidic protein （GFAP） was detected with with ABC immunohistochemical method at hours 6, 24, 48, 72 and day 7 after intervention. RESULTS： There was not GFAP-positive cell found in the sham operation group. The GFAP was little expressed at 6 hours, increased at 48 hours, and reached a peak at 72 hours （P 〈 0.05）. At 7 days, there were still large numbers of GFAP-positive cells, yet it was decreased compared to at 72 hours. The expression of GFAP in the experimental group was identical to that of model group at 6 hours; however, the number of GFAP-positive cells was obvious decreased than model group at the hours 24, 48, 72 and day 7 after intervention （P 〈 0.05）. CONCLUSION： rhG-CSF can inhibit the activation of astrocytes, which may be play an important role in the process of reparation of the nerve tissues and neurofunctional recovery after hemorrhage.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第32期6263-6266,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
泸州医学院2007届优秀硕士课题~~
