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环氧化酶-2在肺纤维化大鼠中的表达及其意义 被引量:3

Signification and expression of cyclooxygenase-2 in rats with bleomycin-induced pulmonary fibrosis
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摘要 目的:观察环氧化酶-2(COX-2)在肺组织中的分布及其抑制剂对博莱霉素(BLM)诱导的大鼠肺纤维化模型的干预作用。方法:雄性SD大鼠72只随机分为3组,分别为对照组、模型组、塞来昔布组。采用免疫组织化学方法检测各组急性肺泡炎期肺部COX-2的表达和分布;HE和Masson染色方法观察肺泡炎和肺纤维化的程度。结果:(1)在肺纤维化早期,模型组和塞来昔布组均可见COX-2的表达,其主要分布在细支气管上皮细胞中,以模型组表达显著。(2)与对照组相比,模型组和塞来昔布组肺泡炎改变明显(P<0.01);塞来昔布干预组的肺泡炎症与模型组比较有所减轻(P<0.05)。(3)于实验第28、42天,模型组和塞来昔布组均可见肺纤维化改变,与对照组比较差异显著(P<0.01);但塞来昔布干预组与模型组比较无显著性差异(P>0.05)。结论:COX-2在肺纤维化早期高度表达,其主要分布在细支气管上皮细胞中;COX-2抑制剂可减轻早期肺泡炎症,对后期肺纤维化无明显抑制作用。 Objective:To investigate the distribution and expression of the cyclooxygenase-2 (COX-2) in lung tissues and the effect of its inhibition in bteomycin (BLM)-induced pulmonary fibrosis in rats.Methods:72 male SD rats were randomly divided into three groups:the control group, model group and celecoxib group. Immunohistochemical method was used to detect the distribution of COX-2 in the stage of acute pulmonary alveolitis. HE and Masson staining methods were used to evaculate the degree of alveolitis and pulmonary fibrosis. Results: ( 1 ) In early stage of pulmonary fibrosis except in the control group, COX-2 expression was found in small bronchial epithelial cells in the other two groups, especially the model group. (2) Compared with the control group, the other two groups had alveolitis( P 〈 0.01 ). Alveolitis in the celecoxib group was hghter than in the BLM group ( P 〈 0. 05). (3) On 28th and 42th day, compared with the control group, the other two groups had polmonary fibrosis. Conclusion: COX-2 had a higher expression in small bronchial epithelial cells in lungs of bleomycin (BLM)-induced pulmonary fibrosis. Cyclooxygenase-2 inhibitor could reduce pulmonary inflammation in early stage, but couldn' t inhibit pulmonary fibrosis in the late stage.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2009年第8期745-747,751,共4页 Chinese Journal of Immunology
基金 吉林省科技厅资助课题(200505133)
关键词 环氧化酶-2 肺纤维化 环氧化酶-2抑制剂 Cyclooxygenase-2 Pulmonary fibrosis Cyclooxygenase inhibitor
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参考文献10

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二级参考文献13

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