摘要
从茄子黄萎病发生严重田块的健株根围土壤中取样，分离出４种对茄黄萎轮枝菌（Ｖｅｒｔｉｃｉｌｉｕｍａｌｂｏａｔｒｕｍ）有抑制作用的细菌。采用划线法在ＰＤＡ平板上选出对致病菌生长具有明显抑制作用的拮抗细菌Ｂ１。培养时间、培养温度对该细菌的拮抗性有很大的影响，在金氏培养液中，３０℃振荡培养３６ｈ，Ｂ１细菌过滤液对致病菌的拮抗性最强，相当于０１８ｍｇ／ｍＬ的多菌灵溶液。Ｂ１细菌还有延缓致病菌孢子萌发和抑制孢子萌发的特性。
Four strains of bacterium which inhibited Verticillium albo-atrum had been screened from eggplant fields. The strain B 1 had obvious inhibition to the pathogen. The antagonistic action of the bacterium B 1 was further tested, the yield of antagonist was affected by temperature and culturing time.The highest inhibiting effect to the pathogen was obtained by culture of King's medium. The inhibition action equaled to 0.18 mg/mL of carbendazim under the condition of 30℃, 36 h by vibrating culture. The antaginist could delay the spores germination of pathogen.
出处
《甘肃农业大学学报》
CAS
CSCD
1998年第2期182-185,共4页
Journal of Gansu Agricultural University
