摘要
以多花黑麦草12个杂交组合的亲本与后代共70个材料,采用SRAP分子标记技术研究杂种与双亲之间的扩增谱带多态性,以甄别真假杂种。结果表明,1)用10对引物组合共得到多态性条带101条,平均每对引物扩增出10.1条多态带,多态性位点百分率为77.1%。表明供试材料在DNA水平上多态性较高,能够很好的揭示材料间的差异,利用SRAP进行品种和杂种鉴定是可行的。2)所鉴定的62个后代中SRAP扩增电泳带型表现出同父、同母、综合和不同于亲本的新带型等多种类型。结合多对引物综合分析所鉴定的62个后代中,有48个后代具有父本的特征带,这48个后代可以鉴定为真杂种。
Using sequence-related amplified polymorphism （SRAP） molecular markers, 70 samples containing 12 annual Loliurn multiflorum hybrid combinations and their progenies were analyzed to study polymorphism and to detect true or false hybrid seeds. 1） Ten primer pairs produced 101 polymorphic bands, averaging 10.1 bands per primer pair. The average percentage of polymorphic bands was 77.1 %. Samples had relatively high molecular genetic polymorphisms so it was feasible to identify the parents and hybrids. 2） Electrophoretic bands of the 62 hybrids identified by SRAP were consistent with results of different hybrid combinations. Progenies with the marker bands of the male but not the female parents were found in 48 progeny which were therefore true hybrids.
出处
《草业学报》
CSCD
北大核心
2009年第4期260-265,共6页
Acta Prataculturae Sinica
基金
科技部973项目(2007CB108907)
国家"十一五"牧草育种攻关项目(2006BAD01A19-9)资助
关键词
多花黑麦草
杂交育种
SRAP
杂种鉴定
Lolium multiflorum
cross breeding
SRAP
hybrid identification
