摘要
目的研究α-MEM、DMEM-LG、RPMI1640三种培养基对Beagle犬骨髓基质细胞(Bonemarrow stromalcells,BMSCs)体外培养生物学行为的影响,为BMSCs的培养寻找合适的培养基。方法取6只Beagle犬的BMSCs,分别用α-MEM、DMEM-LG、RPMI1640三种培养基进行培养,比较3组细胞的贴壁﹑增殖及矿化等方面的差异。结果α-MEM组24h内贴壁的细胞数目最多;消除贴壁差异后,α-MEM和DMEM-LG促增殖作用相近;α-MEM组碱性磷酸酶(Alkaline phosphatase,ALP)表达量最高,但矿化结节形成数目较少,DMEM-LG组矿化结节形成个数最多。结论DMEM-LG培养基较适合BMSCs的培养。
Objective To investigate the effects of different media： α-MEM, DMEM-LG and RPMI 1640 on the biological behavior of bone marrow stromal cells （BMSCs） in Beagle dogs, and provide the more ideal culture medium for BMSCs. Methods BMSCs derived from 6 Beagle dogs were harvested and grown in α-MEM, DMEM-LG and RPMI 1640 media respectively. The proliferation, attachment rate and mineralized nodules of the cells were observed and compared, Results Cells cultured by α-MEM showed the highest attachment rat. After eliminating the difference of adherent rate, there was no obvious difference in the proliferation between α-MEM and DMEM. In α-MEM group, the expression of alkaline phosphate （ALP） was the highest, while the number of mineralized nodules was the least. DMEM group exhibited the most mineralized nodules. Conclusion As a culture medium DMEM-LG may be more suitable for BMSCs of dogs in vitro.
出处
《组织工程与重建外科杂志》
2009年第4期181-185,共5页
Journal of Tissue Engineering and Reconstructive Surgery
基金
国家自然科学基金(30471892)
福建省自然科学基金(2008J0085)
福建医科大学附属口腔医院重点学科建设学术发展基金[闽医大口腔(2008)39号]
关键词
骨髓基质细胞
组织工程
培养基
增殖
分化
Bone marrow stromal ceils
Tissue engineering
Media
Proliferation
Differentiation
