摘要
苯丙氨酸解氨酶(PAL)是苯丙氨酸代谢途径的进入点酶,也是异黄酮合成的关键酶之一。为了分析大豆叶片中异黄酮含量与苯丙氨酸解氨酶(PAL)基因表达量的关系,采用砂培试验,测定了不同生育时期大豆叶片总异黄酮及大豆黄素、黄豆黄素、染料木素的含量,同时测定了相应各生育时期大豆叶片中苯丙氨酸解氨酶(PAL)基因的相对表达量。结果表明:大豆叶片中大豆异黄酮总含量及3种苷元组份含量与叶片中苯丙氨酸解氨酶(PAL)基因的相对表达量有一个协同增减的趋势;在初花期(R1)大豆叶片中的异黄酮总含量和三种苷元组份含量及苯丙氨酸解氨酶(PAL)基因相对表达量均出现一个峰值。
The phenylalanine ammonialyase （PAL）is an access point enzyme and also one of isoflavone synthesis key enzyme in the phenylalanine pathways. In order to study the relationship between the contents of soybean isoflavanones and relative expression quantity of PAL gene, a sand culture experiment was conducted. Soybean leaves at different growth stage were sampled, and the isoflavone content were measured and the relative expression quantity of alanin ammonialyase （PAL）gene were determined by RQ- PCR. There was a coordination fluctuation tendency between phenylalanin ammonialyase （PAL） gene relative expression quantity and isoflavone content in soybean leaf. Soybean isoflavones content and relative expression quantity of phenylalanin ammonialyase（PAL） gene appeared a peak value at R1 stage.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第4期670-673,共4页
Soybean Science
基金
国家科技支撑计划资助项目(2006BAD01A04)
黑龙江"十一五"科技攻关资助项目(GA06B101-1-3)
