摘要
本文建立了以藻红Ｂ（ＴＩＦ）为标记物的非放射性碘标记破伤风类毒素（ＴＴ）的方法。利用ＴＩＦ上的羧基，用氯甲酸异了酯法直接将ＴＩＦ标记于ＴＴ上，经ＵＶ分析，每个ＴＴ分子上平均标记２７个ＴＩＦ分子，ＴＴ的检测范围为１０ｎｇ／ｍｌ～１００μｇ／ｍｌ。
A new nonradioactive iodine labelling method by couplingtetroiodiaflurescein (TIF) directiy to toxoids tetanus (TT) using isobutylchlorocarbonate was established The results showed that 27 molecules of TIF were conjugated to per molecule of TT by UV TT from 10ng/ml～100μg/ml could bedetected
出处
《黑龙江八一农垦大学学报》
1998年第1期74-76,共3页
journal of heilongjiang bayi agricultural university
关键词
电化学免疫
碘标记
破伤风类毒素
Electrochemical Immunoassay
Iodine-labelling
Toxoids Tetanus
