摘要
以柴油为唯一碳源和能源,从南极海水海冰微生物资源库中筛选到一株石油烃低温降解菌希瓦氏菌NJ49,并对影响其生长和降解率的环境因素(pH、温度、盐度、营养盐和表面活性剂)进行了初步研究。结果表明:希瓦氏菌NJ49可作为低温海域石油烃污染生物修复的菌源,其生长和降解的最适条件为:初始pH7.5,温度15℃,盐度6%,摇瓶装量80ml,最佳氮源硝酸铵,最佳磷源为磷酸二氢钾和磷酸氢二钾的混合物,添加表面活性剂可促进希瓦氏菌NJ49的生长和生物降解率。
influence of initial pH, temperature, salty, nutrition and biosurfactant on the growth and degra- dation efficiency of Shewanell sp. NJ49 were assessed. Results revealed that the optimal combination of nutrient constituents and condition to grow and degrade were determined as NH4NO3 , KH2PO4 and K2HP04, NaCl 6%, pH 7.5, 15℃, 80 mL volume. Adding biosurfactant, especially combination negative ion surfactant （SDS） with positive ion surfactant （rhamnolipid） could improve Shewanell sp. N J49 growing and degrading.
出处
《极地研究》
CAS
CSCD
北大核心
2009年第2期81-90,共10页
Chinese Journal of Polar Research
基金
国家自然科学基金项目(40876107)资助
关键词
南极海洋
低温降解菌
柴油
生物降解
环境因素
Antarctic sea, cold-adapted degrading bacteria, diesel, biodegradation, environ-mental factors.
