摘要
目的探讨硫化氢对大鼠肺缺血再灌注氧化损伤的影响。方法以硫化氢钠(NaHS)作为硫化氢供体。50只健康SD大鼠,随机分成假手术组、肺缺血再灌注组及硫氢化钠(NaHS)组,后者进一步分为NaHS10、20、30μmol.kg-13个剂量组;每组10只。NaHS组实验前5d开始每天按体重分别腹腔注射不同剂量的NaHS,实验前15min再次给药;假手术组和肺缺血再灌注组同时同方法给予生理盐水1ml.kg-1。建立大鼠在体肺缺血再灌注模型,实验结束时左心房放血处死大鼠,观察肺组织病理改变,测定肺湿/干重(W/D)值及肺组织匀浆内丙二醛(MDA)、髓过氧化物酶(MPO)含量及超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果肺缺血再灌注组肺组织W/D值、MDA、MPO水平均较假手术组明显升高(P<0.01),而NaHS组上述指标高于假手术组但低于肺缺血再灌注组(均P<0.05);肺缺血再灌注组肺组织SOD、GSH-Px活性较假手术组明显降低(P<0.01),NaHS组上述指标低于假手术组但明显高于肺缺血再灌注组(P<0.05或P<0.01)。上述指标在NaHS3个剂量组间的差异无统计学意义。肺组织病理学检查结果显示,NaHS组肺缺血再灌注损伤减轻。结论硫化氢具有一定的抗大鼠肺缺血再灌注氧化损伤的能力,其作用机制与清除氧自由基及增加内源性抗氧化酶活性有关。
Objective To explore the impact of hydrogen sulfide（H2S） on oxidative damage in lung of rats after ischemia-reperfusion.Methods We used sodium hydrosulfide（NaHS） as the donor of H2S.Fifty SD rats were randomly divided into sham group（n=10）,lung ischemia-reperfusion group（IR group,n=10） and IR＋NaHS group,and the IR＋NaHS group was divided into IR＋NaHS 10μmol·kg-1 group（n=10）,IR＋NaHS 20 μmol·kg-1 group（n=10）,IR＋NaHS 30μmol·kg-1 group（n=10）.Five days before the experiment,rats of IR＋NaHS（10μmol·kg-1,20μmol·kg-1and 30μmol·kg-1） groups were pretreated（intraperitoneal injection） with different dose of NaHS according to the weight,and administration again 15 min before the experiment;sham group and IR group were pretreated（intraperitoneal injection） with normal saline（1ml·kg-1）.An rat lung I/R model was built in vivo.At the end of the experiment,all rats were put to death after blood was collected from left atrium.We examined indices of lung injury：lung histological change,ratio of lung wet weight to dry weight （W/D）,malondialdehyde（MDA） and myeloperoxidase（MPO） content,activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in lung tissues.Results The W/D ratio of lung tissue,contents of MDA and MPO in lung tissue in the IR group and IR＋NaHS groups were significantly higher than those in the sham group （P〈0.05 or P〈0.01）,while the indices above in IR＋NaHS groups were significantly lower than those in the IR group（P〈0.05）;The activities of SOD and GSH-Px in lung tissue in the IR group and IR＋NaHS groups were significantly lower than those in the sham group （P〈0.05 or P〈0.01）,while the indices above in IR＋NaHS groups were significantly higher than those in the IR group（P〈0.05 or P〈0.01）.All the indices abovementioned had no statistical significance in three dose of IR＋NaHS groups.The lung histological change also showed that pre-administration of NaHS could relieve lung ischemia-reperfusion injury.Conclusions These findings suggest that H2S has a certain ability of anti-oxidative damage in lung of rats after ischemia-reperfusion.Its mechanism involves oxyradical elimination and increase the endogenous antioxidant enzyme activity.
出处
《现代医学》
2009年第3期184-188,共5页
Modern Medical Journal
关键词
肺缺血再灌注损伤
硫化氢
硫氢化钠
氧自由基
氧化损伤
lung ischemia reperfusion injury
hydrogen sulfide
sodium hydrosulfide
oxygen free radical
oxidative damage
