摘要
以C57BL/6JSlac小鼠为正常对照组,对自发性Ⅱ型糖尿病动物模型KK-Ay/Ta小鼠的体生长曲线、糖代谢曲线、血清胰岛素、主要脏器重量、脏器系数等部分生物学特性数据进行测试和分析,并对其肾脏、肝脏和胰腺等组织形态学变化进行探讨。结果显示:①KK-Ay/Ta小鼠体重、血糖和血清胰岛素水平比对照组高(P<0.01);②雄性KK-Ay/Ta小鼠与对照组间心脏、胰腺差异显著(P<0.05),肝脏、脾脏、肾脏及雄性的各脂肪系数、雌性的脏器脂肪较对照组差异极显著(P<0.01);③30周龄时KK-Ay/Ta小鼠肾小球内玻璃样变性、血管基底膜增厚、肾小管腔内透明管型;肝脏出现明显脂肪变性,并伴随有肝细胞坏死、炎细胞浸润、纤维组织增生;胰岛数量明显减少、体积缩小、胰岛内细胞数量减少。实验结果证明,KK-Ay/Ta小鼠是一种中高度高血糖、高度胰岛素抵抗,胰岛功能不足和肾脏、肝脏病变,且伴随肥胖的良好Ⅱ型糖尿病动物模型。
To compare the biological characteristics of KK-A^y/Ta mice in growth curve, glucose metabolism, serum insulin, weight of major organs, and organ coefficient with C57BL/6JSlac mice as the control group, then explore the morphologic changes of kidney, liver and pancreas. The result showed： 1.The weight, glucose and serum insulin in KK-A^y/Ta mice were higher than the control group at the same age （P〈0.01）. 2. The heart and pancreas showed significant difference（P〈0.05）. Between KK-A^y/Ta and C57BL/6JSlac male mice, the liver, spleen, kidney, fat showed high significant difference （P〈0.01）. And there were high significant difference in every organ and fat weight of female mice. 3. The glomerulus hyaline degeneration, capillary basement membrane inerassation and hyaline cast in renal tubules were also observed in KK-A^y/Ta. The liver appeared significant adipose degeneration, cell putrescence, inflammatory cell infiltration and fibroplastic proliferation. At the same time, the number of glomeruli pancreatici and islet cells decreased obviously. The results shows that the KK-A^y/Ta mice exhibits hyperglycemia, high insulin resistance, insufficiency of islet function, renal and hepatic alterations, which makes it with obesity as a suitable disease model for studying type 2 diabetes mellitus.
出处
《上海交通大学学报（农业科学版）》
2009年第4期341-345,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
中国科学院战略生物资源技术支撑体系专项(项目编号:CZBZX-1)
