摘要
目的:观察丹芍化纤胶囊治疗对糖尿病肾病(DN)有无改善作用。方法:用链脲佐菌素(STZ)诱导SD大鼠糖尿病模型,每日分别予以依那普利(10 mg/kg)及丹芍化纤胶囊(0.8 g/kg)灌胃治疗,共观察8周。测定各组大鼠血糖、血肌酐、尿肌酐水平及24 h尿蛋白量,光镜下观察大鼠肾组织结构变化。结果:与正常对照组比较,糖尿病组及两治疗组的血糖、血肌酐、尿肌酐等指标明显改变(P<0.05或P<0.01)。丹芍化纤胶囊治疗组和依那普利治疗组大鼠24 h尿蛋白含量显著低于糖尿病模型组(P<0.05)。HE及PAS染色光镜观察显示糖尿病模型组与两治疗组肾脏组织均有不同程度的肾小球基底膜增厚及纤维化,肾小管上皮细胞水变性和坏死,肾间质纤维组织增生及淋巴细胞浸润等病理改变,但两治疗组肾脏组织的损伤程度明显轻于糖尿病肾病模型组。结论:丹芍化纤胶囊治疗可减轻糖尿病肾病大鼠尿蛋白的排出量,并对糖尿病肾脏病变有一定的保护作用。
Objective： To evaluate the effects of Chinese compound preparation, Danshaohuaxian （DSHX） capsule, on diabetic nephropathy （DN） of rats. Methods： Fifty SD male rats were randomly divided into control group （group C）, DN group （group DN）, Enalaprih treated group （group E） and DSHX treated group （ group DS）. DN rat model was prepared by tail vena injection of STZ. Enalaprilt （ 10 mg· kg^-1 · d^- 1 ） and DSHX （0.8 mg · kg^-1· d^- 1 ） were given respectively to groups E and DS orally for eight weeks. The level changes of blood glucose （BG） , serum and urine creatinine （ Scr and Ucr）, 24-hour urine protein （24h UP） were measured and the kidney pathological changes were observed on HE and PAS stained tissue sections with microscope at the end of experiment. Results： BG, Scr, and Ucr levels in groups DN, E and DS were distinctively different from those in group C （P 〈 0.05 or P 〈 0. 01 ）. The excretive quantities of 24 h UP were significantly lower in groups DS and E than that in group DN （ P 〈 0.05 ）. But no significant difference of 24 h UP was found between groups DS and E（P 〉 0.05 ）. Different degrees of glomerular basement membrane thickening and fibration, nephric tubule epithelium cell hydr-degeneration and necrosis, renal interstitium fibroplasias, and lym- phocyte infiltration were found in kidney tissues of groups DN, E and DS. But renal tissue damage in DN group was severer than those in other two treated groups. Conclusion： DSHX capsule can relieve proteinuria and protect renal tissue of DN rats from lesion.
出处
《贵阳医学院学报》
CAS
2009年第4期389-391,395,共4页
Journal of Guiyang Medical College
基金
贵州省社发攻关项目[黔科合SZ字(2008)3049]
关键词
糖尿病
糖尿病肾病
蛋白尿
丹芍化纤胶囊
diabetes mellitus
diabetic nephropathies
proteinuria
Danshaohuaxian proteinuria
