摘要
目的:在肺栓塞疾病的研究中,肺栓塞动物模型具有至关重要的作用,但目前该模型尚存在不足。文中采用可吸收性明胶海绵制作家兔急性肺栓塞(PE)模型,并与数字减影血管造影法(DSA)和CT肺动脉成像(CTPA)及病理进行对照研究,验证该实验影像模型的可行性和准确性。方法:新西兰家兔30只随机分为实验组(n=26)和对照组(n=4)。实验组26只采用明胶海绵栓子经股静脉插管制备成急性PE模型,栓塞前行DSA和CTPA,并于栓塞后2 h、1 d、3 d、7 d再次行CTPA和DSA后处死。对照组4只家兔注入等渗盐水,分别于第3d、7d各处死2只。所有家兔处死后均行病理检查。结果:26只实验组家兔肺栓塞模型24只制备成功,模型制备成功率为92%,24只家兔参与影像及病理分析。在DSA和CTA影像上肺栓塞表现为肺动脉主干及分支的截断,病理检查可见肺动脉内有明胶海绵栓塞物。结论:用明胶海绵作为栓塞物制备家兔急性肺栓塞模型操作简便、成功率高、成本低廉,是肺栓塞影像学研究的一种较好的实验模型。
Objective ： The animal model of pulmonary embolism （PE） has a vital role in the research of pulmonary disease. This study aimed to investigate the feasibility of preparing the rabbit model of acute PE with absorbable gelatin sponge and compare the model with DSA, CTPA and pathology in the studies of PE. Methods： Thirty rabbits were randomly assigned to an experimental group （n = 26） and a control group （n = 4）. The model of acute PE was established in the experimental rabbits with absorbable gelatin sponge through the femoral vena catheter. Pulmonary angiography and CT pulmonary angiography were performed before and after PE formation. The model rabbits were killed at 2 h, 1 d, 3 d and 7 d after DSA and CTPA. The 4 control rabbits were injected with saline solution by the catheter, and killed at 3 d and 7 d. All the rabbits underwent pathological examination. Results ： Twenty-four PE models were successfully established in the 26 experimental rabbits, and the other 2 died due to pulmonary edema, with a success rate of 92%. The obliteration of the PE could be seen on the images of rabbits, and the absorbable gelatin sponge emboli were observed in the pulmonary arteries under the microscope. Conclusion ： The preparation of the rabbit model of acute PE with absorbable gelatin sponge is easy and has a high success rate. It is a good experimental model for the imaging study of PE.
出处
《医学研究生学报》
CAS
2009年第8期811-814,I0002,共5页
Journal of Medical Postgraduates
基金
南京军区南京总医院青年基金课题资助(批准号:Q2008062)
关键词
肺栓塞
体层摄影
X线计算机
肺血管造影术
动物模型
Pulmonary embolism
Tomography, X-ray computer
Pulmonary angiography
Animal model
