摘要
目的：建立筋骨草药材中总环烯醚萜苷的含量测定方法。方法：采用分光光度法,以乙酰哈巴苷（8-acetylharpagide）为对照品,经酸水解、二硝基苯肼乙醇试液-氢氧化钠甲醇水溶液显色后,在586 nm处测定其含量。结果：乙酰哈巴苷及筋骨草药材显色稳定,乙酰哈巴苷在0.016 2-0.324 8 mg范围内线性关系良好（r=0.999 8）,筋骨草药材样品平均回收率为101.2%,RSD=2.0%。结论：建立了比色法测定筋骨草药材中总环烯醚萜苷类含量的方法,该方法简便、准确、重复性好,可作为该药材的质量控制方法。
Objective：To set up a method for the determination of the total iridoid glycoside in herb medicine of Ajuga decumbens Thunb.Methods： Spectrophotometric method was developed to determine the total iridoid glycoside.8-acetylharpagide was used as the reference substance,the samples were hydrolyzed by acid,2,4-dinitrophenylhydrazine ethanol test solution was used as color-developing agent,methanol-water solution of sodium hydroxide as solvent.The maximal absorption wavelength was 586 nm.Results： The absorbance color of 8acetylharpagide and herb sample solutions was stable after the development,there was a good linear relationship in the scope of（0.016 2）～（0.324 8 mg）（r=（0.999 8））.The average recovery of the added reference substance was 101.2% and RSD was 2.0%.Conclusions： The developed method is simple,accurate,reproducible and effective for the quality control of Ajuga decumbens Thunb.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第8期3-5,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技部创新药物和中药现代化专项(2002A2Z3225)
关键词
筋骨草
总环烯醚萜苷
分光光度法
含量测定
Ajuga decumbens Thunb
total iridoid glycoside
spectrophotometry
assay
