摘要
建立了同时分析三聚氰酸和三聚氰胺的反相高效液相色谱法.发现三聚氰酸和三聚氰胺在C8柱上的保留比在C18柱上明显要强,这一结果表明三聚氰酸和三聚氰胺在反相固定相中的保留并非以通常的疏水分配作用为主导.方法的紫外检测下限为0.034～0.31mg/L,标准曲线线性范围为0.5～100mg/L.方法不经特殊的样品前处理即可用于奶粉和游泳池水等样品中三聚氰酸和三聚氰胺的同时分析.
A reversed phase high performance liquid chromatographic method is developed for the simultaneous determination of cyanuric acid and melamine. The retention and separation characteristics of cyanuric acid and melamine on C8 and C18 columns are compared, and the result shows that both of cyanuric acid and melamine are of stronger retention on C8 column than Cls column. This result indicates that the retention mechanism of cyanuric acid and melamine on the reversed phase columns is not controlled by hydrophobic distribution. The detection limits of the cyanuric acid and melamine are 0.034-0.31 mg/L by UV detector, and the linear range of the calibration curves for cyanuric acid and melamine are 0.5-100 mg/L. The proposed method is applied to the simultaneous determination of cyanuric acid and melamine in milk power and swimming pool water without special sample pretreatment.
出处
《中国科学（B辑）》
CSCD
北大核心
2009年第8期845-849,共5页
Science in China(Series B)
关键词
高效液相色谱法
三聚氰酸
三聚氰胺
奶粉
high performance liquid chromatography, cyanuric acid, melamine, milk power
