摘要
目的用PCR-DGGE方法分析乳牙急性根尖周脓肿细菌,以期进一步阐明乳牙急性根尖周脓肿细菌病因学特点。方法采取乳牙急性根尖周脓肿脓液,提取细菌总DNA,采用通用引物对细菌16S rRNA基因的V2-V3区进行扩增,PCR产物进行变性梯度凝胶电泳,切取电泳的条带进行DNA回收,PCR再次扩增后进行克隆测序,鉴定细菌,分析细菌组成。结果乳牙急性根尖周脓肿优势菌的检出从高到低依次是普雷沃菌90.9%,梭杆菌81.8%,消化链球菌63.6%,卟啉单胞菌45.5%,链球菌45.5%,真杆菌45.5%,乳酸杆菌27.3%,弯曲菌27.3%,密螺旋体27.3%,布雷德菌27.3%。结论乳牙急性根尖周脓肿细菌存在个体差异,某些特定细菌以较高频率存在。细菌16S rRNA基因PCR-DGGE,切胶,克隆测序方法除检测出培养法检出的细菌外,还检出难培养的和未预料到的细菌。
Objective To examine the predominant bacteria associated with acute periapical abscesses of deciduous teeth. Methods Pus was collected from 11 children diagnosed as acute abscesses of endodontic origin, and DNA was extracted to evaluate the predominant bacteria by using 16S rRNA PCR-DGGE, cloning and sequence analysis. Results The most prevalent phylotypes of bacteria found were： Prevotella 90. 9% , Fusobacterium 81.8%, Pepto- streptococcus 63.6% , Porphyromonas 45.5% , Streptococcus 45.5% , Eubacterium 45.5% , Lactobacillus 27. 3% , Campylobacter 27.3% ,Treponema 27.3% ,Bulleidia 27.3%. Conclusion The finding of the present study indicates that PCR-DGGE, cloning and sequencing methods could provide additional knowledge regarding the microbiota of acute periradicular abscesses by detecting bacteria that are difficult to grow or unexpected.
出处
《北京口腔医学》
CAS
2009年第4期223-225,共3页
Beijing Journal of Stomatology
基金
国家自然科学基金资助(30572037)
北京市自然科学基金资助(7062028)
