摘要
利用稀释平板法从曲样和窖泥单菌落中分离得到2株毛霉1#和2#,在优化的发酵培养基和发酵条件下产蛋白酶活力分别达到:1#菌6 565 U/g,2#菌9 146 U/g.对两株菌进行紫外诱变,获得的诱变U1#菌、U2#菌分别比出发1#菌、2#菌产蛋白酶活力提高了48.58%和54.45%.
Mucor were isolated by streak platemethod, two Mucor strains were obtained named 1# and 2 e The protease activity of 1# and 2# were 6 565.209 U/g and 9 146.015 U/g respectively. Mutate breeding was made to enhance the producing capability of the strain. U1# compared to 1# , the protease activity increased 48.58 %, U2# compared to 2#, the protease activity increased 54.45 %.
出处
《北京工商大学学报（自然科学版）》
CAS
2009年第4期1-5,共5页
Journal of Beijing Technology and Business University:Natural Science Edition
基金
科技部工业微生物菌种资源标准化整理
整合及共享试点项目(2005DKA21204-07)
关键词
毛霉
分离
紫外诱变
蛋白酶活力
mucor
isolation
mutate breeding
protease activity
